The first two authors contributed equally to this work.
Expression of gangliosides in an immortalized neural progenitor/stem cell line
Article first published online: 14 OCT 2003
Copyright © 2003 Wiley-Liss, Inc.
Journal of Neuroscience Research
Volume 74, Issue 5, pages 769–776, 1 December 2003
How to Cite
Suetake, K., Liour, S. S., Tencomnao, T. and Yu, R. K. (2003), Expression of gangliosides in an immortalized neural progenitor/stem cell line. J. Neurosci. Res., 74: 769–776. doi: 10.1002/jnr.10802
- Issue published online: 17 NOV 2003
- Article first published online: 14 OCT 2003
- Manuscript Accepted: 4 AUG 2003
- Manuscript Revised: 1 AUG 2003
- Manuscript Received: 4 JUN 2003
- NIH. Grant Number: NS11853
- Children's Medical Research Foundation
- neural differentiation;
- neural progenitor cells;
- ganglioside biosynthesis;
- glycolipid subcellular localization;
- membrane microdomain
Glycosphingolipids (GSLs) are known to play important roles in cellular growth and differentiation in the nervous system. The change in expression of gangliosides is correlated with crucial developmental events and is evolutionarily conserved among many vertebrate species. The emergence of neural progenitors represents a crucial step in neural development, but little is known about the exact composition and subcellular localization of gangliosides in neural progenitor cells. The C17.2 cell line was derived after v-myc transformation of neural progenitor cells isolated from neonatal mouse cerebellar cortex. The developmental potential of C17.2 cells is similar to that of endogenous neural progenitor/stem cells in that they are multipotential and capable of differentiating into all neural cell types. We characterized the GSL composition of C17.2 cells and found the presence of only a-series gangliosides. Subcellular localization studies revealed that GM1 and GD1a are localized mainly on the plasma membrane and partly in the cytoplasm, both as punctate clusters. Reverse transcription-polymerase chain reaction revealed the absence of ST-II transcripts in C17 cells, which most likely accounts for the lack of expression of b- and c-series complex gangliosides in this cell line. These data suggest that the divergence in ganglioside expression in C17.2 cells is regulated at the transcriptional level. © 2003 Wiley-Liss, Inc.