Functional alterations in immature cultured rat hippocampal neurons after sustained exposure to static magnetic fields
Version of Record online: 1 DEC 2003
Copyright © 2003 Wiley-Liss, Inc.
Journal of Neuroscience Research
Volume 75, Issue 2, pages 230–240, 15 January 2004
How to Cite
Hirai, T. and Yoneda, Y. (2004), Functional alterations in immature cultured rat hippocampal neurons after sustained exposure to static magnetic fields. J. Neurosci. Res., 75: 230–240. doi: 10.1002/jnr.10819
- Issue online: 30 DEC 2003
- Version of Record online: 1 DEC 2003
- Manuscript Accepted: 12 AUG 2003
- Manuscript Revised: 30 JUL 2003
- Manuscript Received: 25 APR 2003
- Ministry of Education, Culture, Sports, Science and Technology, Japan
- Ministry of Health, Labour and Welfare, Japan
- static magnetic fields;
In cultured rat hippocampal neurons, gradual increases were seen in the expression of microtubule-associated protein-2 (MAP-2), neuronal nuclei (NeuN) and growth-associated protein-43 (GAP-43), in proportion to increased duration, up to 9 days in vitro (DIV). Sustained exposure to static magnetic fields at 100 mT for up to 9 DIV significantly decreased expression of MAP-2 and NeuN in cultured rat hippocampal neurons without markedly affecting GAP-43 expression. Although a significant increase was seen in the expression of glial fibrillary acidic protein (GFAP) in hippocampal neuronal preparations cultured for 6–9 DIV under sustained magnetism, GFAP and proliferating cell nuclear antigen expression were not affected markedly in cultured astrocytes prepared from rat hippocampus and neocortex, irrespective of cellular maturity. No significant alteration was seen in cell survivability of hippocampal neurons or astrocytes cultured under sustained magnetism. In hippocampal neurons cultured for 3 DIV under sustained magnetism, marked mRNA expression was seen for N-methyl-D-aspartate (NMDA) receptor subunits, NR1, NR2A-2C, NR2D, and NR3A. In addition, significant potentiation of the ability of NMDA to increase intracellular free Ca2+ ions was observed. Differential display analysis revealed a significant decrease in mRNA expression for the transcription factor ALF1 in response to sustained magnetism for 3 DIV. These results suggest that sustained exposure to static magnetic fields may affect cellular functionality and maturity in immature cultured rat hippocampal neurons through modulation of expression of particular NMDA receptor subunits. © 2003 Wiley-Liss, Inc.