Estrogen stimulates brain-derived neurotrophic factor expression in embryonic mouse midbrain neurons through a membrane-mediated and calcium-dependent mechanism
Article first published online: 28 SEP 2001
Copyright © 2001 Wiley-Liss, Inc.
Journal of Neuroscience Research
Volume 66, Issue 2, pages 221–230, 15 October 2001
How to Cite
Ivanova, T., Küppers, E., Engele, J. and Beyer, C. (2001), Estrogen stimulates brain-derived neurotrophic factor expression in embryonic mouse midbrain neurons through a membrane-mediated and calcium-dependent mechanism. J. Neurosci. Res., 66: 221–230. doi: 10.1002/jnr.1214
- Issue published online: 28 SEP 2001
- Article first published online: 28 SEP 2001
- Manuscript Accepted: 20 JUN 2001
- Manuscript Revised: 22 MAY 2001
- Manuscript Received: 4 MAR 2001
- DFG. Grant Numbers: SFB 497, projects B2 and A5
- Medical Clinic of the University of Ulm. Grant Number: P.544
- growth factors
We have provided evidence that 17β-estradiol (E) synthesized in the midbrain promotes the differentiation of midbrain dopamine neurons through nonclassical steroid action. Because these developmental effects resemble those reported for brain-derived neurotrophic factor (BDNF), we hypothesized that E influences dopaminergic cell differentiation through a BDNF-dependent mechanism. Competitive RT-PCR and ELISA techniques were employed to study first the developmental pattern of BDNF and trkB expression in the mouse midbrain. BDNF protein/mRNA levels peaked postnatally, whereas trkB did not fluctuate perinatally. To prove the hypothesis that E regulates BDNF expression in vivo, fetuses and newborns were treated with the aromatase antagonist CGS 16949A. CGS 16949A exposure reduced midbrain BDNF mRNA/protein levels. The coapplication of CGS 16949A and E abolished this effect. Midbrain cultures from mouse fetuses were used to investigate intracellular signaling mechanisms involved in transmitting E effects. Estrogen increased expression of BDNF but not of other neurotrophins. As concerns the related signaling mechanism, these effects were antagonized by interrupting intracellular Ca2+ signaling with BAPTA and thapsigargin but not by the estrogen receptor antagonist ICI 182,780. Insofar as E effects on BDNF mRNA expression were inhibited by cycloheximide, it appears likely that other, not yet characterized intermediate proteins take part in the estrogenic regulation of BDNF expression. We conclude that E exerts its stimulatory effect on the differentiation of dopaminergic neurons by coordinating BDNF expression. This particular E effect appears to be transmitted through Ca2+-dependent signaling cascades upon activation of putative membrane estrogen receptors. J. Neurosci. Res. 66:221–230, 2001. © 2001 Wiley-Liss, Inc.