Regional estimates of hair cells and supporting cells in the human crista ampullaris

Authors

  • Ivan Lopez,

    1. Surgery Department, Division of Head and Neck, David Geffen School of Medicine at UCLA,Los Angeles, California
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  • Gail Ishiyama,

    1. Neurology Department, David Geffen School of Medicine at UCLA, Los Angeles, California
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  • Yong Tang,

    1. Department of Histology and Embryology, Chongqing University of Medical Sciences,Peoples Republic of China
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  • Joshua Tokita,

    1. Surgery Department, Division of Head and Neck, David Geffen School of Medicine at UCLA,Los Angeles, California
    2. University of Illinois at Chicago College of Medicine, Chicago, Illinois
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  • Robert W. Baloh,

    1. Surgery Department, Division of Head and Neck, David Geffen School of Medicine at UCLA,Los Angeles, California
    2. Neurology Department, David Geffen School of Medicine at UCLA, Los Angeles, California
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  • Akira Ishiyama

    Corresponding author
    1. Surgery Department, Division of Head and Neck, David Geffen School of Medicine at UCLA,Los Angeles, California
    • CHS 62-132, UCLA School of Medicine, 10833 Le Conte Avenue, Los Angeles, CA 90095
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Abstract

Regional estimates of type I and type II vestibular hair cells (HC) and supporting cell (SC) numbers were obtained from the horizontal crista ampullaris by using design-based stereology in human. Cristae were microdissected from temporal bones obtained post-mortem (N = 16, age range 26–98 years). Three groups were made according to age: group 1, n = 5, ages between 26 and 67 years, average age 51 years; group 2, n = 4, average age 84 years; and group 3, n = 7, average age 94 years. For group 1, the average total HC number was 8,005 ± 214, corresponding to 4,119 ± 107 type I HC, 3,886 ± 117 type II HC, and 10,274 ± 224 SC. The type I:type II HC ratio was 1.06 ± 0.01, and HC density was 0.80 cells/100 μm2. For group 2, the average total HC number was 7,074 ± 489, corresponding to 3,733 ± 212 type I HC, 3,341 ± 314 type II HC, and 9,321 ± 858 SC. The type I:II HC ratio was 1.12 ± 0.06, and HC density was 0.75 cells/100 μm2. For group 3, the average HC number was 6,009 ± 327, corresponding to 3,380 ± 223 type I HC, 2,628 ± 235 type II HC, and 10,185 ± 182 SC. The type I:II HC ratio was 1.34 ± 0.10, and HC density was 0.63 cells/100 μm2. A significant decline in type I, type II, and total HC number and density was found in groups 2 and 3, with individuals exceeding the average human life span. © 2005 Wiley-Liss, Inc.

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