Neuroprotective effect of N-acetylcysteine on neuronal apoptosis induced by a synthetic gingerdione compound: Involvement of ERK and p38 phosphorylation
Article first published online: 18 SEP 2006
Copyright © 2006 Wiley-Liss, Inc.
Journal of Neuroscience Research
Volume 84, Issue 7, pages 1485–1494, 15 November 2006
How to Cite
Lin, C.-H., Kuo, S.-C., Huang, L.-J. and Gean, P.-W. (2006), Neuroprotective effect of N-acetylcysteine on neuronal apoptosis induced by a synthetic gingerdione compound: Involvement of ERK and p38 phosphorylation. J. Neurosci. Res., 84: 1485–1494. doi: 10.1002/jnr.21047
- Issue published online: 17 OCT 2006
- Article first published online: 18 SEP 2006
- Manuscript Accepted: 10 JUL 2006
- Manuscript Revised: 13 JUN 2006
- Manuscript Received: 17 APR 2006
- National Science Council of Taiwan. Grant Numbers: NSC93-2320-B-039-024, NSC94-2752-B-006-001-PAE
- extra-cellular signal-regulated kinases;
- P38 MAPK;
Besides being used as a spice, ginger has been applied in oriental medicine to ameliorate symptoms such as inflammatory, rheumatic disorders, and gastrointestinal discomforts. The effects of ginger on neuronal cells, however, have not been explored. We investigate the effect of 1-(3,4-dimethoxyphenyl)-3,5-dodecenedione (I6), a derivative of gingerdione, on cultured cortical neurons. After a 5-day maturation period in vitro, cortical neurons were treated with I6 for 24 hr and cell viability was assessed using MTT assay. I6 induced neuronal death in a concentration-dependent manner. Hoechst 33342, propidium iodide (PI), and TUNEL staining confirmed that the reduced cell viability by I6 was due to apoptosis. Pre-treatment of cell with N-acetylcysteine (NAC) prevented cell death in a concentration-dependent manner. N-acetylcysteine increased phosphorylated levels of p42 and p44 extracellular signal-regulated kinases (ERKs). In parallel, farnesyltransferase and MEK inhibitors blocked ERK phosphorylation and neuroprotective effect of NAC. Unexpectedly, NAC also increased phosphorylated level of p38 mitogen-activated protein kinase (MAPK) and p38 specific inhibitors dose-dependently attenuated the effect of NAC. Farnesyltransferase and MEK inhibitors completely abolished NAC-induced p38 phosphorylation whereas p38 inhibitor did not influence NAC-induced ERK phosphorylation. These results show that NAC serially activates ERKs and p38 MAPK, and ERKs and p38 work together to mediate the neuroprotective effect of NAC. © 2006 Wiley-Liss, Inc.