Research Article

“Soluble” adenylyl cyclase-generated cyclic adenosine monophosphate promotes fast migration in PC12 cells

  1. Jennifer J. Young1,†,
  2. Amna Mehdi2,†,
  3. Lori L. Stohl2,
  4. Lonny R. Levin3,*,
  5. Jochen Buck3,
  6. John A. Wagner2,
  7. Alexander M. Stessin4

Article first published online: 6 AUG 2007

DOI: 10.1002/jnr.21458

Issue

Journal of Neuroscience Research

Journal of Neuroscience Research

Volume 86, Issue 1, pages 118–124, January 2008

Additional Information

How to Cite

Young, J. J., Mehdi, A., Stohl, L. L., Levin, L. R., Buck, J., Wagner, J. A. and Stessin, A. M. (2008), “Soluble” adenylyl cyclase-generated cyclic adenosine monophosphate promotes fast migration in PC12 cells. Journal of Neuroscience Research, 86: 118–124. doi: 10.1002/jnr.21458

Author Information

  1. 1

    Gateways to the Laboratory Program, Weill Medical College of Cornell University, New York, New York

  2. 2

    Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, New York

  3. 3

    Department of Pharmacology, Weill Medical College of Cornell University, New York, New York

  4. 4

    Tri-Institutional MD/PhD Program, Weill Medical College of Cornell University, New York, New York

  1. †

    The first two authors contributed equally to this work.

*Department of Pharmacology, Weill Medical College of Cornell University, New York, NY 10026

Publication History

  1. Issue published online: 10 DEC 2007
  2. Article first published online: 6 AUG 2007
  3. Manuscript Revised: 7 JUN 2007
  4. Manuscript Accepted: 7 JUN 2007
  5. Manuscript Received: 18 MAY 2007

Funded by

  • MSTP (to A.M.S.)
  • NIH. Grant Numbers: HD42060 (to J.B.), GM62328 (to J.B.), AI64842, NS55255
  • American Diabetes Association
  • Hirschl Weil-Caulier Trust (to L.R.L.)
  • Milstein Foundation (to J.B. and L.R.L.)

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Keywords:

  • adenylyl cyclase;
  • nerve growth factor;
  • neuron;
  • compartmentalization;
  • protein kinase C

Abstract

In a model for neuronal movement, PC12 cells undergo fast migration in response to nerve growth factor (NGF) and phorbol ester (PMA). We previously showed that NGF increases intracellular cAMP via activation of soluble adenylyl cyclase (sAC). In this report, we demonstrate that sAC activation is an essential component of NGF- + PMA-induced fast migration in PC12 cells. Interestingly, PMA also raises intracellular cAMP but does so by stimulating transmembrane adenylyl cyclases (tmAC); however, this tmAC-generated cAMP does not contribute to fast migration. Therefore, cells must possess independent pools of cAMP capable of modulating distinct functions. © 2007 Wiley-Liss, Inc.

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