Functional recovery after spinal cord injury in mice through activation of microglia and dendritic cells after IL-12 administration
Version of Record online: 25 APR 2008
Copyright © 2008 Wiley-Liss, Inc.
Journal of Neuroscience Research
Volume 86, Issue 9, pages 1972–1980, July 2008
How to Cite
Yaguchi, M., Ohta, S., Toyama, Y., Kawakami, Y. and Toda, M. (2008), Functional recovery after spinal cord injury in mice through activation of microglia and dendritic cells after IL-12 administration. J. Neurosci. Res., 86: 1972–1980. doi: 10.1002/jnr.21658
- Issue online: 16 JUN 2008
- Version of Record online: 25 APR 2008
- Manuscript Accepted: 18 DEC 2007
- Manuscript Revised: 15 DEC 2007
- Manuscript Received: 7 JUL 2007
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
- spinal cord injury;
- dendritic cells;
We have previously reported that the transplantation of dendritic cells (DCs) brings about functional recovery after spinal cord injury in mice through the activation of endogenous microglia/macrophages and neural stem/progenitor cells. In this study, the effect of interleukin-12 (IL-12), which is secreted from DCs, was evaluated for the treatment of spinal cord injury in mice. Administration of IL-12 into the injured site significantly increased the number of activated microglia/macrophages and DCs as well as the expression of brain-derived neurotrophic factor surrounding the lesion site. Immunohistochemical analyses showed that de novo neurogenesis and remyelination were induced by IL-12 treatment. Furthermore, an open field test using Basso-Beattie-Brenham scoring revealed a significant improvement of locomotor function in mice treated with IL-12. These results suggest that IL-12 administration into the injured spinal cord results in a functional recovery through the activation of microglia/macrophages and DCs. © 2008 Wiley-Liss, Inc.