The first two authors contributed equally to this work.
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Research Article
Specificity of the second messenger pathways involved in basic fibroblast growth factor-induced survival and neurite growth in chick ciliary ganglion neurons
Article first published online: 29 APR 2009
DOI: 10.1002/jnr.22116
Copyright © 2009 Wiley-Liss, Inc.
Additional Information
How to Cite
Gilardino, A., Farcito, S., Zamburlin, P., Audisio, C. and Lovisolo, D. (2009), Specificity of the second messenger pathways involved in basic fibroblast growth factor-induced survival and neurite growth in chick ciliary ganglion neurons. J. Neurosci. Res., 87: 2951–2962. doi: 10.1002/jnr.22116
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The first two authors contributed equally to this work.
Publication History
- Issue published online: 12 AUG 2009
- Article first published online: 29 APR 2009
- Manuscript Accepted: 17 MAR 2009
- Manuscript Revised: 16 MAR 2009
- Manuscript Received: 20 AUG 2008
Funded by
- Compagnia di San Paolo Foundation (to the Centre of Excellence on Nanostructured Surfaces and Interfaces)
- Abstract
- Article
- References
- Cited By
Keywords:
- fibroblast growth factor-2 (FGF-2);
- neurite outgrowth;
- neuronal survival;
- signal transduction pathways;
- ciliary ganglion neurons
Abstract
Basic fibroblast growth factor (bFGF) exerts multiple neurotrophic actions on cultured neurons from the ciliary ganglion of chick embryo, among them promotion of neuronal survival and of neurite outgrowth. To understand the specificity of the signal transduction cascades involved in the control of these processes, we used pharmacological inhibitors of the three main effectors known to act downstream of the bFGF receptor (FGFR): phospholipase Cγ (PLCγ), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3-K). Neuronal survival was assessed at 24 and 48 hr; neurite growth was analyzed both on dissociated neurons and on explants of whole ganglia. Our data show that only the PI3-K pathway is involved in the survival-promoting effect of bFGF; on the other hand, all three effectors converge on the enhancement of neurite outgrowth, both on isolated neurons and in whole ganglia. © 2009 Wiley-Liss, Inc.