Glial cell line-derived neurotrophic factor enhances in vitro differentiation of mid-/hindbrain neural progenitor cells to dopaminergic-like neurons
Article first published online: 20 SEP 2010
Copyright © 2010 Wiley-Liss, Inc.
Journal of Neuroscience Research
Volume 88, Issue 15, pages 3222–3232, 15 November 2010
How to Cite
Young, A., Assey, K. S., Sturkie, C. D., West, F. D., Machacek, D. W. and Stice, S. L. (2010), Glial cell line-derived neurotrophic factor enhances in vitro differentiation of mid-/hindbrain neural progenitor cells to dopaminergic-like neurons. J. Neurosci. Res., 88: 3222–3232. doi: 10.1002/jnr.22499
- Issue published online: 11 OCT 2010
- Article first published online: 20 SEP 2010
- Manuscript Accepted: 20 JUL 2010
- Manuscript Revised: 19 JUL 2010
- Manuscript Received: 19 JAN 2010
- GRA Eminent Endowment
- DOD. Grant Number: N000140810989
Additional Supporting Information may be found in the online version of this article.
|JNR_22499_sm_suppfig1.tif||2961K||Supporting Information Figure 1. Neurons differentiated with GDNF express distinct neural subtype populations. CHAT, a marker for cholinergic neurons, gene expression was seen in neurons differentiated with or without GDNF for 21 days (A). Neurons differentiated with GDNF for 21 days also expressed PMNT, a marker for adrenergic neurons (A), DBH, a marker for noradrenergic neurons, (A) and GAD, a marker for gluatmatergic neurons (A). DBH was also expressed in hNPs as well as in differentiated neurons without GDNF for 21 days (E). Markers for gabaergic neurons (GIRK, A) and serotenergic neurons (TPH, A) were not seen in any cell type. Immunocytochemistry for DBH and TH in neurons differentiated for 14 days with GDNF demonstrate a subpopulation that express TH only (B, C, D green arrows) and a subpopulation that express DBH and TH (B, C, D red arrows). CHAT - choline acetyltransferase; GIRK - G protein-coupled inwardly rectifying potassium channel; TPH - tryptophan hydroxylase 1; PMNT - phenylethanolamine-N-methyl transferase; GAD - glutamate dehydroxylase; GDNF - glial cell-line derived neurotropic factor; DBH – dopamine beta hydroxylase; TH – tyrosine hydroxylase; GDNF – glial cell-line derived neurotrophic factor. Scale bars = 10μM.|
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