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JNR_22499_sm_suppfig1.tif2961KSupporting Information Figure 1. Neurons differentiated with GDNF express distinct neural subtype populations. CHAT, a marker for cholinergic neurons, gene expression was seen in neurons differentiated with or without GDNF for 21 days (A). Neurons differentiated with GDNF for 21 days also expressed PMNT, a marker for adrenergic neurons (A), DBH, a marker for noradrenergic neurons, (A) and GAD, a marker for gluatmatergic neurons (A). DBH was also expressed in hNPs as well as in differentiated neurons without GDNF for 21 days (E). Markers for gabaergic neurons (GIRK, A) and serotenergic neurons (TPH, A) were not seen in any cell type. Immunocytochemistry for DBH and TH in neurons differentiated for 14 days with GDNF demonstrate a subpopulation that express TH only (B, C, D green arrows) and a subpopulation that express DBH and TH (B, C, D red arrows). CHAT - choline acetyltransferase; GIRK - G protein-coupled inwardly rectifying potassium channel; TPH - tryptophan hydroxylase 1; PMNT - phenylethanolamine-N-methyl transferase; GAD - glutamate dehydroxylase; GDNF - glial cell-line derived neurotropic factor; DBH – dopamine beta hydroxylase; TH – tyrosine hydroxylase; GDNF – glial cell-line derived neurotrophic factor. Scale bars = 10μM.

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