Cell–extracellular matrix versus cell–cell interactions during the development of the cochlear-vestibular ganglion
Version of Record online: 6 MAY 2011
Copyright © 2011 Wiley-Liss, Inc.
Journal of Neuroscience Research
Volume 89, Issue 9, pages 1375–1387, September 2011
How to Cite
Davies, D. (2011), Cell–extracellular matrix versus cell–cell interactions during the development of the cochlear-vestibular ganglion. J. Neurosci. Res., 89: 1375–1387. doi: 10.1002/jnr.22664
- Issue online: 6 JUL 2011
- Version of Record online: 6 MAY 2011
- Manuscript Accepted: 17 MAR 2011
- Manuscript Revised: 31 JAN 2011
- Manuscript Received: 1 OCT 2010
- Royal National Institute for the Deaf (RNID). Grant Number: G17
- BBSRC. Grant Number: BB/E002927/1
Additional Supporting Information may be found in the online version of this article.
|JNR_22664_sm_suppinfoFig1.tif||17564K||Supporting Information Fig. 1. Identification of cell types in otocyst culture. Confocal micrographs showing expression of an epithelial marker, E-cadherin (red), and neuroblast marker, βIII tubulin (green) in a ventral otocyst preparation after 24 hr in culture. E-cadherin is concentrated in the epithelium (B,C,H,I) with very little staining associated with neuroblasts (B,C, E,F). βIII tubulin is highly expressed in neuroblasts (A,C,D,F) ans is expressed at much lower levels in the epihtelium (A,C,G, I). Scale bars = 100 μm. E, epithelium; N, neuroblasts|
|JNR_22664_sm_suppinfoFig2.tif||3452K||Supporting Information Fig. 2: Measurement of otic ganglion volume. The confocal micrographs show an example of an optical section through the cultured whole otocyst, highlighted by DAPI-stained nuclei, and the ganglion that formed during the incubation period, identified by the presence of NGN-1 GFP positive neuroblasts. The ganglion area measured for this section is highlighted with a dotted line. Equivalent measurements were made for each optical section and combined to calculate volume of the ganglion as described in Materials and Methods. O, otocyst; E, epithelium; G, ganglion. Scale bar = 100 μm|
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