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Additional Supporting Information may be found in the online version of this article.

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JNR_22733_sm_SuppMov1.avi2189KSupplemental Videos 1 and 2. Time-lapse microscopy of GFP-MBP-C1-UTR (Video 1), and of GFP-MBP-C1-T92E-UTR (Video 2; green), over a 4 hr duration, showing oscillations in protein trafficking from the nucleus to the cytoplasm. Images were acquired at intervals of 6 min after establishing a steady baseline (for 3 hr) following cell transfer to the live-cell imaging environment.
JNR_22733_sm_SuppMov2.avi2854KSupplemental Videos 1 and 2. Time-lapse microscopy of GFP-MBP-C1-UTR (Video 1), and of GFP-MBP-C1-T92E-UTR (Video 2; green), over a 4 hr duration, showing oscillations in protein trafficking from the nucleus to the cytoplasm. Images were acquired at intervals of 6 min after establishing a steady baseline (for 3 hr) following cell transfer to the live-cell imaging environment.
JNR_22733_sm_SuppMov3.avi26584KSupplemental Videos 3 and 4. Time-lapse microscopy of N19 OLGs expressing GFP-MBP-C1-UTR (Video 3) and coexpressing GFP-MBP-C1-UTR with constitutively active Fyn (p59Fyn-Y527F; Video 4) over a 9 hr duration showing N19-OLG branching morphology, and cell motility. Individual cells were tracked and paths are provided at different points during the time course. Scale bar = 50 μm. Images were acquired at intervals of 6 min and 10 min after establishing a steady baseline (for 3 hr) following cell culture transfer to the live-cell imaging environment, respectively. Significant differences in membrane branching and elaboration can be observed between cells expressing MBP-C1-UTR with and without constitutively active Fyn. Scale bar = 50 μm.
JNR_22733_sm_SuppMov4.avi14383KSupplemental Videos 3 and 4. Time-lapse microscopy of N19 OLGs expressing GFP-MBP-C1-UTR (Video 3) and coexpressing GFP-MBP-C1-UTR with constitutively active Fyn (p59Fyn-Y527F; Video 4) over a 9 hr duration showing N19-OLG branching morphology, and cell motility. Individual cells were tracked and paths are provided at different points during the time course. Scale bar = 50 μm. Images were acquired at intervals of 6 min and 10 min after establishing a steady baseline (for 3 hr) following cell culture transfer to the live-cell imaging environment, respectively. Significant differences in membrane branching and elaboration can be observed between cells expressing MBP-C1-UTR with and without constitutively active Fyn. Scale bar = 50 μm.
JNR_22733_sm_SuppFig1.eps10275KSupplementary Figure 1. Fluorescence micrographs of cultured N19-OLGs, 2 days posttransfection, over-expressing different wild-type and variant versions of GFP-tagged MBP-C1-UTR (green), coexpressing different versions of wild-type (p59Fyn), inactive (p59Fyn-K299M), or constitutively active Fyn (p59Fyn-Y527F; red). The Fyn protein was immunostained and detected using secondary Alexa594-conjugated antibodies, along with nuclei counterstained with DAPI (blue). Images were acquired using a 10X objective to capture a substantial number of cells to demonstrate that morphological differences were consistent throughout the population of cultured N19-OLGs.

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