High doses of salicylate and aspirin are inhibitory on acid-sensing ion channels and protective against acidosis-induced neuronal injury in the rat cortical neuron
Version of Record online: 4 OCT 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Neuroscience Research
Volume 90, Issue 1, pages 267–277, January 2012
How to Cite
Wang, W., Ye, S.-D., Zhou, K.-Q., Wu, L.-M. and Huang, Y.-N. (2012), High doses of salicylate and aspirin are inhibitory on acid-sensing ion channels and protective against acidosis-induced neuronal injury in the rat cortical neuron. J. Neurosci. Res., 90: 267–277. doi: 10.1002/jnr.22742
- Issue online: 15 NOV 2011
- Version of Record online: 4 OCT 2011
- Manuscript Accepted: 20 JUN 2011
- Manuscript Revised: 16 JUN 2011
- Manuscript Received: 1 DEC 2010
- Anhui Provincial Natural Science Foundation. Grant Number: 090413103
- Anhui Provincial Hospital Affiliated to Anhui Medical University
Additional Supporting Information may be found in the online version of this article.
|JNR_22742_sm_SuppFig1.tif||10K||Supplementary Figure 1. The acid-induced ASIC-like current in the cultured cerebral cortical neuron of the rat. (A) left, Representative traces showing that transient inward currents were pH-dependently elicited; right, The dose-response curve for activation of the currents by decreasing extracellular pH. Each point represents the average response of 4–6 neurons. All responses were normalized to the peak current induced by extracellular pH 6.0 (I6.0, *). The horizontal bars indicate the drug application duration and the vertical bars show the mean ± SEM; the VH was –50 mV. (B) Three typical types of transient inward currents induced by extracellular pH 6.0 solutions. left, The transient current almost completely desensitized (type □ current); middle, The transient current followed by slowly desensitizing current (type □ current); right, The transient current followed by a sustained current (type □ current). (C) left, Representative traces showing that the acid-induced transient currents were reversibly inhibited by 10 and 100 μM amiloride (AMI), respectively. right, The dose-response curve for inhibition of the amplitude of pH 6.0-induced transient currents by amiloride. Each point represents the average response of 5 neurons. All responses were normalized to the I6.0. (D) Representative traces showing that APETx2 (3 μM; a) and PcTX-1 (20 nM; b) reversibly inhibited the amplitude of acid-induced transient current, respectively.|
|JNR_22742_sm_SuppFig2.tif||4K||Supplementary Figure 2.|
|JNR_22742_sm_SuppFig3.tif||323K||Supplementary Figure 3.|
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