N. Carkaci-Salli and S. Battula contributed equally to this work.
Gender-specific regulation of tyrosine hydroxylase in thymocyte differentiation antigen-1 knockout mice
Article first published online: 19 MAR 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Neuroscience Research
Volume 90, Issue 8, pages 1583–1588, August 2012
How to Cite
Carkaci-Salli, N., Battula, S., Wang, X., Connor, J.R. and Vrana, K.E. (2012), Gender-specific regulation of tyrosine hydroxylase in thymocyte differentiation antigen-1 knockout mice. J. Neurosci. Res., 90: 1583–1588. doi: 10.1002/jnr.23046
- Issue published online: 6 JUN 2012
- Article first published online: 19 MAR 2012
- Manuscript Accepted: 21 JAN 2012
- Manuscript Revised: 19 JAN 2012
- Manuscript Received: 22 JUN 2011
- NIH. Grant Number: R01GM-38931
- PA Department of Health
- tyrosine hydroxylase;
- gender-specific expression
Thymocyte differentiation antigen-1 (Thy-1) is a cell surface glycoprotein found on T cells and neurons and is involved in cell-to-cell interactions. In addition, Thy-1 knockouts (KO) are a potential mouse model of restless legs syndrome (RLS) based on clinical observations and the role of dopamine in the disease. In this study, we analyzed the activity and quantity of tyrosine hydroxylase (TH; the rate-limiting enzyme in dopamine production) and determined phosphorylation levels for the enzyme phosphoserine-40 (pSer-40). There was no significant difference in the total TH activity and pSer-40 TH levels between Thy-1 KO and control groups; however, TH specific activity was significantly lower (by 26%) in Thy-1 KO mice. This difference is due in part to increased TH protein levels in this group (increased by 29%). When analyzed by gender, Thy-1 KO female mouse striata contained less TH specific activity compared with control females (decreased by 41%) and male control or Thy-1 KO animals (decreased by 30%). TH specific activity and pSer-40 TH levels in male Thy-1 KO and control displayed no differences. However, pSer-40 TH was significantly higher in control females (38%) compared with control or Thy-1 KO males. The Thy-1 KO females exhibited significantly lower (28%) pSer-40 TH (normalized to GAPDH or TH) than control females. Indeed, the Thy-1 KO females had 50% of the pSer-40 TH found in controls. Our results suggest a gender effect on TH specific activity, TH protein levels, and serine-40 phosphorylation of TH in Thy-1 KO female mice. © 2012 Wiley Periodicals, Inc.