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Olig2-lineage cells preferentially differentiate into oligodendrocytes but their processes degenerate at the chronic demyelinating stage of proteolipid protein-overexpressing mouse

Authors

  • Takahiro Shimizu,

    1. Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Kanagawa, Japan
    2. Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki, Japan
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  • Kenji F. Tanaka,

    1. Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Kanagawa, Japan
    2. Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki, Japan
    3. Department of Neuropsychiatry, School of Medicine, Keio University, Tokyo, Japan
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  • Hirohide Takebayashi,

    1. Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Kanagawa, Japan
    2. Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki, Japan
    3. Institute of Medicine and Dentistry, Sensory and Integrative Medicine, Biological Functions and Medical Control, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
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  • Mikito Higashi,

    1. Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki, Japan
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  • Wilaiwan Wisesmith,

    1. Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Kanagawa, Japan
    2. Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki, Japan
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  • Katsuhiko Ono,

    1. Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Kanagawa, Japan
    2. Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki, Japan
    3. Department of Biology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan
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  • Seiji Hitoshi,

    1. Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Kanagawa, Japan
    2. Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki, Japan
    3. Department of Integrative Physiology, Shiga University of Medical Science, Shiga, Japan
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  • Kazuhiro Ikenaka

    Corresponding author
    1. Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Kanagawa, Japan
    2. Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki, Japan
    • Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan
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Abstract

In chronic demyelinating lesions of the central nervous system, insufficient generation of oligodendrocytes (OLs) is not due to a lack of oligodendrocyte precursor cells (OPCs), because the accumulation of OPCs and premyelinating OLs can be observed within these lesions. Here we sought to identify the basis for the failure of OLs to achieve terminal differentiation in chronic demyelinating lesions through the utilization of plp1-overexpressing (Plp tg/−) mice. These mice are characterized by progressive demyelination in young adults and chronic demyelinating lesions at more mature stages. We show that neural stem cells, which are the precursors of OL-lineage cells, are present in the Plp tg/− mouse brain and that their multipotentiality and ability to self-renew are comparable to those of wild-type adults in culture. Lineage-tracing experiments using a transgenic mouse line, in which an inducible Cre recombinase is knocked in at the Olig2 locus, revealed that Olig2-lineage cells preferentially differentiated into OPCs and premyelinating OLs, but not into astrocytes, in the Plp tg/− mouse brain. These Olig2-lineage cells matured to express myelin basic protein but after that their processes degenerated in the chronic demyelinating lesions of the Plp tg/− brain. These results indicate that in chronic demyelinated lesions more OL-lineage cells are produced as part of the repair process, but their processes degenerate after maturation. © 2012 Wiley Periodicals, Inc.

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