Deceased June 1, 2011.
Article first published online: 27 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Neuroscience Research
Volume 91, Issue 3, pages 321–334, March 2013
How to Cite
Gopalakrishnan, G., Awasthi, A., Belkaid, W., De Faria, O., Liazoghli, D., Colman, D. R. and Dhaunchak, A. S. (2013), Lipidome and proteome map of myelin membranes . J. Neurosci. Res., 91: 321–334. doi: 10.1002/jnr.23157
Deceased August 18, 2012.
- Issue published online: 16 JAN 2013
- Article first published online: 27 NOV 2012
- Manuscript Accepted: 20 SEP 2012
- Manuscript Revised: 17 SEP 2012
- Manuscript Received: 29 MAY 2012
- Government of Canada (to MNI)
- Rio Tinto Alcan
- The Molson Foundation
- Myelin Repair Foundation
- MS Society of Canada (to A.S.D.)
- multiple sclerosis;
To understand the molecular anatomy of myelin membranes, we performed a large-scale, liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS)-based lipidome and proteome screen on freshly purified human and murine myelin fractions. We identified more than 700 lipid moieties and above 1,000 proteins in the two species, including 284 common lipids and 257 common proteins. This study establishes the first comprehensive map of myelin membrane components in human and mice. Although this study demonstrates many similarities between human and murine myelin, several components have been identified exclusively in each species. Future quantitative validation studies focused on interspecies differences will authenticate the myelin membrane anatomy. The combined lipidome and proteome map presented here can nevertheless be used as a reference library for myelin health and disease. © 2012 Wiley Periodicals, Inc.