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Lipidome and proteome map of myelin membranes

Authors

  • Gopakumar Gopalakrishnan,

    Corresponding author
    1. Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
    2. Department of Chemistry, McGill University, Montreal, Quebec, Canada
    3. McGill Program in Neuroengineering, McGill University, Montreal, Quebec, Canada
    • Montreal Neurological Institute/Department of Chemistry, McGill Program in Neuroengineering, McGill University, Montreal, Quebec, H3A 0B8 Canada
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  • Anshul Awasthi,

    1. Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
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  • Wiam Belkaid,

    1. Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
    2. McGill Program in Neuroengineering, McGill University, Montreal, Quebec, Canada
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  • Omar De Faria Jr.,

    1. Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
    2. McGill Program in Neuroengineering, McGill University, Montreal, Quebec, Canada
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  • Dalinda Liazoghli,

    1. Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
    2. McGill Program in Neuroengineering, McGill University, Montreal, Quebec, Canada
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  • David R. Colman,

    1. Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
    2. McGill Program in Neuroengineering, McGill University, Montreal, Quebec, Canada
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  • Ajit S. Dhaunchak

    1. Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
    2. McGill Program in Neuroengineering, McGill University, Montreal, Quebec, Canada
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  • Deceased June 1, 2011.

  • Deceased August 18, 2012.

Abstract

To understand the molecular anatomy of myelin membranes, we performed a large-scale, liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS)-based lipidome and proteome screen on freshly purified human and murine myelin fractions. We identified more than 700 lipid moieties and above 1,000 proteins in the two species, including 284 common lipids and 257 common proteins. This study establishes the first comprehensive map of myelin membrane components in human and mice. Although this study demonstrates many similarities between human and murine myelin, several components have been identified exclusively in each species. Future quantitative validation studies focused on interspecies differences will authenticate the myelin membrane anatomy. The combined lipidome and proteome map presented here can nevertheless be used as a reference library for myelin health and disease. © 2012 Wiley Periodicals, Inc.

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