Zebrafish brain proteomics reveals central proteins involved in neurodegeneration
Article first published online: 5 OCT 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Neuroscience Research
Volume 92, Issue 1, pages 104–115, January 2014
How to Cite
Gebriel, M., Prabhudesai, S., Uleberg, K.-E., Larssen, E., Piston, D., Bjørnstad, A. H. and Møller, S. G. (2014), Zebrafish brain proteomics reveals central proteins involved in neurodegeneration. J. Neurosci. Res., 92: 104–115. doi: 10.1002/jnr.23297
- Issue published online: 21 NOV 2013
- Article first published online: 5 OCT 2013
- Manuscript Accepted: 12 AUG 2013
- Manuscript Revised: 5 AUG 2013
- Manuscript Received: 10 MAY 2013
- Regional Development Programme grant (RUP) from The Rogaland County
- Norwegian Research Council
- Western Health Authority in Norway
Understanding the complex biology of the brain requires analyzing its structural and functional complexity at the protein level. The large-scale analysis of the brain proteome, coupled with characterization of central brain proteins, provides insight into fundamental brain processes and processes linked to neurodegenerative diseases. Here we provide a map of the zebrafish brain proteome by using two-dimensional gel electrophoresis (2DE), followed by the identification of 95 brain proteins using mass spectrometry (LC-ESI MS/MS). Our data show extensive phosphorylation of brain proteins but less prominent glycosylation. Furthermore, ∼51% of the identified proteins are predicted to have one or more ubiquitination sites whereas ∼90% are predicted to have one or more SUMOylation sites. Our findings provide a valuable proteome map of the zebrafish brain and associated posttranslational modifications demonstrating that zebrafish proteomic approaches can aid in our understanding of proteins central to important neuronal processes and those associated with neurodegenerative disorders. © 2013 Wiley Periodicals, Inc.