J. Tomàs, M. Santafé, N. Garcia, and M.A. Lanuza contributed equally to this work.
Presynaptic membrane receptors in acetylcholine release modulation in the neuromuscular synapse
Version of Record online: 27 JAN 2014
Copyright © 2014 Wiley Periodicals, Inc.
Journal of Neuroscience Research
Volume 92, Issue 5, pages 543–554, May 2014
How to Cite
Tomàs, J., Santafé, M. M., Garcia, N., Lanuza, M. A., Tomàs, M., Besalduch, N., Obis, T., Priego, M. and Hurtado, E. (2014), Presynaptic membrane receptors in acetylcholine release modulation in the neuromuscular synapse. J. Neurosci. Res., 92: 543–554. doi: 10.1002/jnr.23346
- Issue online: 12 MAR 2014
- Version of Record online: 27 JAN 2014
- Manuscript Accepted: 19 NOV 2013
- Manuscript Revised: 11 NOV 2013
- Manuscript Received: 11 JUL 2013
- MEC . Grant Number: SAF2011-23711
- Catalan Government (Generalitat) . Grant Number: 2009SGR01248 (to J.T.).
- purinergic receptors;
- muscarinic receptors;
- neurotrophin receptors;
- cytokine receptors
Over the past few years, we have studied, in the mammalian neuromuscular junction (NMJ), the local involvement in transmitter release of the presynaptic muscarinic ACh autoreceptors (mAChRs), purinergic adenosine autoreceptors (P1Rs), and trophic factor receptors (TFRs; for neurotrophins and trophic cytokines) during development and in the adult. At any given moment, the way in which a synapse works is largely the logical outcome of the confluence of these (and other) metabotropic signalling pathways on intracellular kinases, which phosphorylate protein targets and materialize adaptive changes. We propose an integrated interpretation of the complementary function of these receptors in the adult NMJ. The activity of a given receptor group can modulate a given combination of spontaneous, evoked, and activity-dependent release characteristics. For instance, P1Rs can conserve resources by limiting spontaneous quantal leak of ACh (an A1R action) and protect synapse function, because stimulation with adenosine reduces the magnitude of depression during repetitive activity. The overall outcome of the mAChRs seems to contribute to upkeep of spontaneous quantal output of ACh, save synapse function by decreasing the extent of evoked release (mainly an M2 action), and reduce depression. We have also identified several links among P1Rs, mAChRs, and TFRs. We found a close dependence between mAChR and some TFRs and observed that the muscarinic group has to operate correctly if the tropomyosin-related kinase B receptor (trkB) is also to operate correctly, and vice versa. Likewise, the functional integrity of mAChRs depends on P1Rs operating normally. © 2014 Wiley Periodicals, Inc.