B. Luo, J. Huang, and L. Lu contributed equally to this work.
Electrically induced brain-derived neurotrophic factor release from schwann cells
Version of Record online: 19 FEB 2014
Copyright © 2014 Wiley Periodicals, Inc.
Journal of Neuroscience Research
Volume 92, Issue 7, pages 893–903, July 2014
How to Cite
Luo, B., Huang, J., Lu, L., Hu, X., Luo, Z. and Li, M. (2014), Electrically induced brain-derived neurotrophic factor release from schwann cells. J. Neurosci. Res., 92: 893–903. doi: 10.1002/jnr.23365
- Issue online: 13 MAY 2014
- Version of Record online: 19 FEB 2014
- Manuscript Accepted: 27 DEC 2013
- Manuscript Revised: 18 DEC 2013
- Manuscript Received: 3 JUL 2013
- National Natural Science Foundation of China. Grant Numbers: 81201389, 30973052, 30872870
- National Basic Research Program of China (973 Program). Grant Number: 2014CB542206
- Program for Changjiang Scholar and Innovative Research Team in University. Grant Number: IRT1053
- electrical stimulation;
- brain-derived neurotrophic factor;
- calcium-calmodulin dependent protein kinase;
- mitogen-activated protein kinase
Regulating the production of brain-derived neurotrophic factor (BDNF) in Schwann cells (SCs) is critical for their application in traumatic nerve injury, neurodegenerative disorders, and demyelination disease in both central and peripheral nervous systems. The present study investigated the possibility of using electrical stimulation (ES) to activate SCs to release BDNF. We found that short-term ES was capable of promoting BDNF production from SCs, and the maximal BDNF release was achieved by ES at 6 V (3 Hz, 30 min). We further examined the involvement of intracellular calcium ions ([Ca2+]i) in the ES-induced BDNF production in SCs by pharmacological studies. We found that the ES-induced BDNF release required calcium influx through T-type voltage-gated calcium channel (VGCC) and calcium mobilization from internal calcium stores, including inositol triphosphate-sensitive stores and caffeine/ryanodine-sensitive stores. In addition, calcium-calmodulin dependent protein kinase IV (CaMK IV), mitogen-activated protein kinase (MAPK), and cAMP response element-binding protein (CREB) were found to play important roles in the ES-induced BDNF release from SCs. In conclusion, ES is capable of activating SCs to secrete BDNF, which requires the involvement of calcium influx through T-type VGCC and calcium mobilization from internal calcium stores. In addition, activation of CaMK IV, MAPK, and CREB were also involved in the ES-induced BDNF release. The findings indicate that ES can improve the neurotrophic ability in SCs and raise the possibility of developing electrically stimulated SCs as a source of cell therapy for nerve injury in both peripheral and central nervous systems. © 2014 Wiley Periodicals, Inc.