Get access

Mulberroside a protects against ischemic impairment in primary culture of rat cortical neurons after oxygen–glucose deprivation followed by reperfusion

Authors

  • Cai-Ping Wang,

    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, China
    Search for more papers by this author
    • C.-P. Wang and L.-Z. Zhang contributed equally to this work

  • Lu-Zhong Zhang,

    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, China
    Search for more papers by this author
    • C.-P. Wang and L.-Z. Zhang contributed equally to this work

  • Gui-Cai Li,

    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, China
    Search for more papers by this author
  • Yun-wei Shi,

    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, China
    Search for more papers by this author
  • Jian-Long Li,

    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, China
    Search for more papers by this author
  • Xiao-Chuan Zhang,

    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, China
    Search for more papers by this author
  • Zhi-Wei Wang,

    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, China
    2. Department of Pharmacology, University of California, Irvine, California
    Search for more papers by this author
  • Fei Ding,

    Corresponding author
    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, China
    • Correspondence to: Fei Ding, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, No. 19, Qixiu Road, Nantong 226001, People's Republic of China. E-mail: shengdd@public.nt.js.cn; Xin-Miao Liang, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, People's Republic of China. E-mail: liangxm@dicp.ac.cn

    Search for more papers by this author
  • Xin-Miao Liang

    Corresponding author
    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, China
    2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
    • Correspondence to: Fei Ding, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, No. 19, Qixiu Road, Nantong 226001, People's Republic of China. E-mail: shengdd@public.nt.js.cn; Xin-Miao Liang, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, People's Republic of China. E-mail: liangxm@dicp.ac.cn

    Search for more papers by this author

Abstract

Mulberroside A is a natural polyhydroxylated stilbene compound present at relatively high abundance in the roots and twigs of Morus alba L. It is known for its nephroprotective, hypoglycemic, and antidiabetic effects. Because its metabolite, oxyresveratrol, possessed purported anti-inflammatory and neuroprotective effects, we proposed that mulberroside A may elicit neuroprotective effects that can be used in the treatment of brain ischemic injury. Therefore, we decided to investigate the pharmacological properties of mulberroside A in primary culture of rat cortical neurons after oxygen–glucose deprivation followed by reperfusion (OGD/R), evaluating its ability to counteract the hypoxia–ischemia impairment. The results showed that mulberroside A elicited neuroprotective effects comparable to nimodipine. The mechanistic studies showed that mulberroside A decreased the expressions of tumor necrosis factor-α (TNF-α), interleukin (IL)−1β, and IL-6 and inhibited the activation of NALP3, caspase-1, and nuclear factor-κB and the phosphorylation of extracellular signal-regulated protein kinases, the c-Jun N-terminal kinase, and p38, exhibiting anti-inflammatory antiapoptotic effects. Our results also further demonstrate that the proinflammatory cytokines of IL-1β, IL-6, and TNF-α are promising targets for treatment of cerebral ischemic injury. Although further investigation is required for its development, all of these findings led us to speculate that mulberroside A is a candidate for the treatment of ischemic stroke, which would act as a multifactorial neuroprotectant. © 2014 Wiley Periodicals, Inc.

Get access to the full text of this article

Ancillary