S-180 cells secrete nerve growth factor protein similar to 7S-nerve growth factor
Article first published online: 11 OCT 2004
Copyright © 1981 Alan R. Liss, Inc.
Journal of Neuroscience Research
Volume 6, Issue 1, pages 21–36, 1981
How to Cite
Barklis, E. and Perez-Polo, J. R. (1981), S-180 cells secrete nerve growth factor protein similar to 7S-nerve growth factor. J. Neurosci. Res., 6: 21–36. doi: 10.1002/jnr.490060104
- Issue published online: 11 OCT 2004
- Article first published online: 11 OCT 2004
- Manuscript Accepted: 4 NOV 1980
- Manuscript Received: 11 AUG 1980
- mouse sarcoma;
- high-molecular-weight complexes
The nerve growth factor protein (NGF) has been identified by biological assay and rocket immunoelectrophoresis in media conditioned by monolayers of mouse S-180 sarcoma cells, a transformed cell line of salivary gland origin. By utilization of a purification procedure designed to enrich for acid-dissociable, high-molecular-weight complexes similar to the 7S-NGF isolated from male mouse submaxillary gland, it has been determined that a significant portion of mouse sarcoma NGF is initially present as a complex at neutral pH with a molecualr weight indistinguishable from that of 7S-NGF. At pH 4.0 the mouse sarcoma NGF complex dissociates, and the active subunit can be isolated as a species with a molecular weight of less than 40,000. The induced dissociation at pH 4.0 of the mouse sarcoma NGF high-molecular-weight complex, as well as the complex's behavior in isoelectric focusing and sucrose gradient sedimentation is consistent with the hypothesis that 7S-NGF is packaged as a subunit containing protein intracellularly prior to secretion into the extracellular space. Moreover, the stability of the mouse sarcoma NGF complex at dilute concentrations is similar to that reported for the purified 7S-NGF complex.