Depolarizing agents and tumor necrosis factor-α modulate protein phosphorylation in oligodendrocytes
Version of Record online: 11 OCT 2004
Copyright © 1994 Wiley-Liss, Inc.
Journal of Neuroscience Research
Volume 38, Issue 1, pages 91–100, 1 May 1994
How to Cite
Soliven, B., Takeda, M. and Szuchet, S. (1994), Depolarizing agents and tumor necrosis factor-α modulate protein phosphorylation in oligodendrocytes. J. Neurosci. Res., 38: 91–100. doi: 10.1002/jnr.490380112
- Issue online: 11 OCT 2004
- Version of Record online: 11 OCT 2004
- Manuscript Received: 20 OCT 1993
- Manuscript Accepted: 16 FEB 1993
- Manuscript Revised: 27 JAN 1993
- ion channels;
- myelin basic protein;
Membrane depolarization and changes in ionic fluxes have been implicated in the signaling mechanisms between neurons and glial cells. We report here that K+-induced depolarization of cultured ovine oligodendrocytes (OLGs) decreases the phosphorylation of myelin basic protein (MBP) and 2′3′-cyclic nucleotide phosphohydrolase (CNPase). Membrane depolarization and decrease in phosphorylation of MBP and CNPase can also be elicited by inhibition of the inward rectifier with Ba2+ but not by inhibition of outward K+ channels with 4-aminopyridine or tetraethylammonium. These findings demonstrate that modulation of K+ currents can influence phosphorylation states of OLG proteins. Tumor necrosis factor-α (TNF-α), an immune peptide implicated in autoimmune demyelinating diseases, also inhibits the phosphorylation of these proteins. In contrast to elevated [K+]0, TNF-α does not decrease the stimulatory effect of protein kinase C activators or phosphatase inhibitors on MBP and CNPase phosphorylation, suggesting that depolarizing agents and TNF-α act via distinct mechanisms. We postulate that the presence of elevated extracellular K+ and/or cytokines under certain pathological conditions can perturb OLG function by altering the phosphorylation states of their proteins and perhaps affect myelin maintenance, contributing to demyelination. © 1994 Wiley-Liss, Inc.