Experimental allergic encephalomyelitis: Characterization of T lymphocytes that bind myelin basic protein and synapsin
Article first published online: 19 NOV 2004
Copyright © 1996 Wiley-Liss, Inc.
Journal of Neuroscience Research
Volume 43, Issue 1, pages 50–54, 1996
How to Cite
de Santis, M. L. and Roth, G. A. (1996), Experimental allergic encephalomyelitis: Characterization of T lymphocytes that bind myelin basic protein and synapsin. J. Neurosci. Res., 43: 50–54. doi: 10.1002/jnr.490430106
- Issue published online: 19 NOV 2004
- Article first published online: 19 NOV 2004
- Manuscript Accepted: 14 JUN 1995
- Manuscript Revised: 2 JUN 1995
- Manuscript Received: 18 MAY 1995
The immuno phenotypic profile of the mononuclear cells that bind myelin basic protein (MBP) and synapsin was investigated in lymph node cells from rats with experimental allergic encephalomyelitis induced by injection with MBP. Using a double immunofluorescent labeling technique, purified cells that bind one or both antigens were analyzed in different stages of the disease. The total MBP-bound lymphocytes increased at 14 days post-inoculation (dpi), had a CD4+/CD8+ ratio of two and were present until 29 dpi. Conversely, the apportionment of cells specific for MBP that also recognize synapsin reached a maximum value at 14 dpi coincidentally with the expression of the paralysis symptoms and then, they disappeared when the animal began to recover. This population amounted to about 40% of the total lymph node MBP-bound cells and had a CD4+/CD8+ ratio of one, indicating that the lymphocytes with MBPsynapsin crossreactivity could be principally implicated in a cytotoxic or suppressor activity. © 1996 Wiley-Liss, Inc.