This research tested a multilevel model examining the influence of work-family culture and supportive workplace relationships on work interference with family. Web-based survey data were provided by 792 information technology employees from 10 organizations. Random coefficient modeling was used to test a path model examining the relationships between work-family culture, leader-member exchange (LMX), coworker support, and work interference with family. The direct effects of LMX and coworker support on work interference with family were significant. The indirect effect of work-family culture on work interference with family was also significant. Results demonstrate the value of work-family culture in understanding supportive supervisory and coworker relationships and work interference with family and highlight the need to employ multilevel models to understand these relationships. Copyright © 2007 John Wiley & Sons, Ltd.