Biosynthesis of poly-3-hydroxybutyric acid by the facultatively methanol-assimilating bacterium Mycoplana rubra B346 and recombinant strains

Authors

  • Christina G. Foóllner,

    1. Fakultät für Biowissenschaften, Pharmazie und Psychologie der Universität Leipzig; Abteilung Biotechnologie, Permoserstraße 15, D-04318 Leipzig, Germany
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  • Susi Müuller,

    1. Fakultät für Biowissenschaften, Pharmazie und Psychologie der Universität Leipzig; Abteilung Biotechnologie, Permoserstraße 15, D-04318 Leipzig, Germany
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  • Alexander Steinbüchel,

    1. Institut für Mikrobiologie der West-fälischen Wilhelms-Universität Münster, Corrensstraße 3, D-48149 Münster, Germany
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  • Wolfgang Babel

    Corresponding author
    1. Umwelt-forschungszentrum Leipzig-Halle GmbH, Sektion Umweltmikrobiologie, Permoserstraße 15, D-04318 Leipzig, Germany
    • Umweltforschungszentrum Leipzig-Halle GmbH, Sektion Umweltmikrobiologie, Permoserstr. 15, 04318 Leipzig, Germany
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Abstract

Mycoplana rubra B346 is a pink-pigmented facultatively methylotrophic bacterium which is capable of synthesizing and accumulating small amounts of poly-3-hydroxybutyric acid (PHB) if a carbon source (e.g. methanol) is provided in excess and if an essential nutrient (e.g. nitrogen or phoshorus, respectively) is limiting growth. Upon transfer of the PHA-biosynthesis genes of Alcaligenes eutrophus H16 or Chromatium vinosum D or of the isolated PHA-synthase structural gene of Methylobacterium extorquens into M. rubra B346, the content of PHB increased from 9.6 up to 25% of the cell dry matter. M. rubra and some recombinant strains were characterized physiologically and morphologically, and the size of the cells and the PHB content were compared by flow cytometry.

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