Chemicals and heat generate different protein patterns in Acinetobacter calcoaceticus

Authors

  • Dirk Benndorf,

    1. Umweltforschungszentrum Leipzig-Halle GmbH, Sektion Umweltmikrobiologie, PF2, 04301 Leipzig, Germany
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  • Norbert Loffhagen,

    1. Umweltforschungszentrum Leipzig-Halle GmbH, Sektion Umweltmikrobiologie, PF2, 04301 Leipzig, Germany
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  • Prof. Dr. Wolfgang Babel

    Corresponding author
    1. Umweltforschungszentrum Leipzig-Halle GmbH, Sektion Umweltmikrobiologie, PF2, 04301 Leipzig, Germany
    • UFZ-Umweltforschungszentrum Leipzig-Halle GmbH, Sektion Umweltmikrobiologie, PF 2, 04301 Leipzig, Germany
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Abstract

The effect of exposing Acinetobacter calcoaceticus 69-V to DNP1-stress and heat shock was examined by two-dimensional gel electrophoresis of proteins, which were detected either by autoradiography or by silver staining. Both DNP stress and heat shock led to altered patterns of protein synthesis or concentration. About 10% of the proteins which were synthesized newly or at an increased rate and about 25% of those which were found newly or with an increased concentration after DNP treatment were identified after heat shock, too.

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