Research Articles

Use of a collagen-platelet rich plasma scaffold to stimulate healing of a central defect in the canine ACL

  1. Martha M. Murray1,*,
  2. Kurt P. Spindler2,
  3. Clint Devin2,
  4. Brian S. Snyder3,
  5. John Muller3,
  6. Masaya Takahashi3,
  7. Percy Ballard1,2,
  8. Lillian B. Nanney4,
  9. David Zurakowski1

Article first published online: 22 MAR 2006

DOI: 10.1002/jor.20073

Issue

Journal of Orthopaedic Research

Journal of Orthopaedic Research

Volume 24, Issue 4, pages 820–830, April 2006

Additional Information

How to Cite

Murray, M. M., Spindler, K. P., Devin, C., Snyder, B. S., Muller, J., Takahashi, M., Ballard, P., Nanney, L. B. and Zurakowski, D. (2006), Use of a collagen-platelet rich plasma scaffold to stimulate healing of a central defect in the canine ACL. Journal of Orthopaedic Research, 24: 820–830. doi: 10.1002/jor.20073

Author Information

  1. 1

    Department of Orthopaedic Surgery, Children's Hospital of Boston, 300 Longwood Avenue, Boston, Massachusetts 02115

  2. 2

    Vanderbilt Sports Medicine, Department of Orthopaedic Surgery and Rehabilitation, Vanderbilt University Medical Center, Nashville, Tennessee 37232

  3. 3

    Orthopaedic Biomechanics Laboratory, Beth Israel Deaconess Hospital, Boston, Massachusetts 02115

  4. 4

    Department of Plastic Surgery, Vanderbilt University Medical Center, Nashville, Tennessee 37203

*Department of Orthopaedic Surgery, Children's Hospital of Boston, 300 Longwood Avenue, Boston, Massachusetts 02115. Telephone: 617-355-7497; Fax: 617-730-0459

Publication History

  1. Issue published online: 22 MAR 2006
  2. Article first published online: 22 MAR 2006
  3. Manuscript Accepted: 15 AUG 2005
  4. Manuscript Received: 17 JUN 2005

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Keywords:

  • anterior cruciate ligament;
  • wound healing;
  • canine;
  • platelets;
  • collagen

Abstract

The anterior cruciate ligament (ACL) of the knee fails to heal after primary repair. Here we hypothesize that a beneficial biologic repair response can be induced by placing a collagen-platelet rich plasma (collagen-PRP) material into a central ACL defect. A collagen-PRP scaffold was used to treat a central ACL defect in vivo. In the first experiment, the histologic response in treated and untreated defects was evaluated at 3 (n = 5) and 6 weeks (n = 5). In the second experiment, biomechanical testing of the treated ligaments (n = 8) was performed at 6 weeks and compared with the results of biomechanical testing of untreated defects at the same time-point (n = 6). The percentage filling of the defects in the treated ACLs was significantly higher at both the 3- and 6-week time-points when compared with the untreated contralateral control defects (50 ± 21% vs. 2 ± 2% at 3 weeks, and 43 ± 11% vs. 23 ± 11 at 6 weeks; all values mean ± SEM. Biomechanically, the treated ACL defects had a 40% increase in strength at 6 weeks, which was significantly higher than the 14% increase in strength previously reported for untreated defects (p < 0.02). Placement of a collagen-PRP bridging scaffold in a central ACL defect can stimulate healing of the ACL histologically and biomechanically. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 24:820–830, 2006

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