Gene expression analysis of major lineage-defining factors in human bone marrow cells: Effect of aging, gender, and age-related disorders

Authors

  • Ying Jiang,

    1. Nanotechnology Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba Ibaraki, 305-8566, Japan
    2. Fudan University, Shanghai, People's Rebublic of China
    3. Medical School of Nantong University, Jiangsu, People's Republic of China
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  • Hajime Mishima,

    1. Department of Orthopedic Surgery, Control of Musculoskeletal Systems, Advanced Biomedical Applications, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 2-1-1 Tennodai, Tsukuba Ibaraki, 305-8572, Japan
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  • Shinsuke Sakai,

    1. Department of Orthopedic Surgery, Control of Musculoskeletal Systems, Advanced Biomedical Applications, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 2-1-1 Tennodai, Tsukuba Ibaraki, 305-8572, Japan
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  • Yin-kun Liu,

    1. Fudan University, Shanghai, People's Rebublic of China
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  • Yoshimi Ohyabu,

    1. Nanotechnology Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba Ibaraki, 305-8566, Japan
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  • Toshimasa Uemura

    Corresponding author
    1. Nanotechnology Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba Ibaraki, 305-8566, Japan
    2. Tokyo Medical and Dental University, 1-5-45 Yushima Bunkyo-ku, Tokyo, 113-8519, Japan
    • Nanotechnology Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba Ibaraki, 305-8566, Japan. Telephone: 81-29-861-2724; Fax 81-29-861-2789.
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Abstract

Adult bone marrow cells (BMCs) include two populations:;mesenchymal stem cells (MSCs), which can differentiate into bone, cartilage, and fat; and hematopoietic stem cells (HSCs), which produce all mature blood lineage. To study the effect of aging, gender, and age-related disorders on lineage differentiation, we performed quantitative RT-PCR to examine mRNA expression of the major factors defining BMC lineage, cbfa1 for osteoblasts, ppar-gamma for adipocytes, sox9 for chondrocytes, and rankl for osteoclasts, in bone marrow from 80 healthy subjects and patients (14–79 years old) with two age-related disorders: osteoarthritis (OA) and rheumatoid arthritis (RA). Two apoptosis-related genes, bcl-2 and drak1, were studied. RANKL and PPAR-Gamma levels exhibited a clear positive correlation with age in female patients, but not in males, with a slight age-related decline in CBFa1 transcripts. DRAK1 expression showed an age-associated ascending trend with significantly greater transcripts of RANKL and DRAK1 in females (p < 0.01). Compared with age-matched controls, RA patients exhibited increased RANKL, PPAR-Gamma, and DRAK1 mRNA levels (p < 0.05), and OA showed the higher RANKL and PPAR-Gamma transcripts (p < 0.05). Furthermore, SOX9 and DRAK1 expressions in the RA group were higher than in the OA group (p < 0.05). Our data indicate that aging and age-related disorders affect gene expressions differently, suggesting that in aging, the lineage of bone marrow cells was modified with prominent changes in decreased bone marrow osteoblastogenesis, increased adipogenesis and osteoclastogenesis, while in age-related disorders, marrow adipogenesis and the activity or number of osteoclasts may play an important role in the pathogenesis of arthritic bone loss. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:910–917, 2008

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