Research Article

Ciprofloxacin up-regulates tendon cells to express matrix metalloproteinase-2 with degradation of type I collagen

  1. Wen-Chung Tsai1,
  2. Chih-Chin Hsu2,
  3. Carl P.C. Chen1,
  4. Hsiang-Ning Chang1,
  5. Alice M.K. Wong1,
  6. Miao-Sui Lin1,
  7. Jong-Hwei S. Pang3,*

Article first published online: 2 JUL 2010

DOI: 10.1002/jor.21196

Issue

Journal of Orthopaedic Research

Journal of Orthopaedic Research

Volume 29, Issue 1, pages 67–73, January 2011

Additional Information

How to Cite

Tsai, W.-C., Hsu, C.-C., Chen, C. P.C., Chang, H.-N., Wong, A. M.K., Lin, M.-S. and Pang, J.-H. S. (2011), Ciprofloxacin up-regulates tendon cells to express matrix metalloproteinase-2 with degradation of type I collagen. J. Orthop. Res., 29: 67–73. doi: 10.1002/jor.21196

Author Information

  1. 1

    Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Linkou, College of Medicine, Chang Gung University, Linkou, Taiwan

  2. 2

    Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Keelung, College of Medicine, Chang Gung University, Taiwan

  3. 3

    Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taiwan

*Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Linkou, College of Medicine, Chang Gung University, Linkou, Taiwan. T: 886-3-2118800, ext. 3482; F: 886-3-3280170.

Publication History

  1. Issue published online: 22 NOV 2010
  2. Article first published online: 2 JUL 2010
  3. Manuscript Accepted: 19 MAY 2010
  4. Manuscript Received: 22 JAN 2010

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (242K)

Keywords:

  • tendon;
  • ciprofloxacin;
  • matrix metalloproteinase;
  • collagen

Abstract

Ciprofloxacin-induced tendinopathy and tendon rupture have been previously described, principally affecting the Achilles tendon. This study was designed to investigate the effect of ciprofloxacin on expressions of matrix metalloproteinases (MMP)-2 and -9, tissue inhibitors of metalloproteinase (TIMP)-1 and -2 as well as type I collagen in tendon cells. Tendon cells intrinsic to rat Achilles tendon were treated with ciprofloxacin and then underwent MTT (tetrazolium) assay. Real-time reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis were used, respectively, to evaluate the gene and protein expressions of type I collagen, and MMP-2. Gelatin zymography was used to evaluate the enzymatic activities of MMP-2 and -9. Reverse zymography was used to evaluate TIMP-1 and -2. Immunohistochemical staining for MMP-2 in ciprofloxacin-treated tendon explants was performed. Collagen degradation was evaluated by incubation of conditioned medium with collagen. The results revealed that ciprofloxacin up-regulated the expression of MMP-2 in tendon cells at the mRNA and protein levels. Immunohistochemistry also confirmed the increased expressions of MMP-2 in ciprofloxacin-treated tendon explants. The enzymatic activity of MMP-2 was up-regulated whereas that of MMP-9, TIMP-1 or TIMP-2 was unchanged. The amount of secreted type I collagen in the conditioned medium decreased and type I collagen was degraded after ciprofloxacin treatment. In conclusion, ciprofloxacin up-regulates the expressions of MMP-2 in tendon cells and thus degraded type I collagen. These findings suggest a possible mechanism of ciprofloxacin-associated tendinopathy. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:67–73, 2011

View Full Article (HTML) Get PDF (242K)