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Keywords:

  • bifocal microscopy;
  • DPD;
  • leaf bronzing;
  • Oryza sativa L.;
  • selection

Abstract

A protocol for a novel method to visualize Fe(II) in rice tissues is proposed. The method is based on the selective formation of a purple-red color complex of 2,2′ bipyridine and Fe(II). Rice genotypes were exposed to 18 mM Fe(II) in nutrient solution for 2 d. Root systems of intact plants were subsequently placed in 2,2′ bipyridine solutions. The formation of the [Fe(bipy)<$>_3^{2+}<$>] color complex was visualized using bifocal microscopy. The method may improve the selection of genotypes during breeding for Fe-toxicity resistance of rice.