This study examines the relation of differentiation of self in the workplace to work stress and work satisfaction among individuals working in various employment settings. Family theorist Murray Bowen (1976, 1978) defined differentiation as encompassing one's ability to attain a mature balance between autonomy and intimacy within relationships. The purpose of this study was to determine whether better levels of differentiation would translate into better workplace relationships that would reflect in greater job satisfaction and lower perceived stress levels. The Emotional Reactivity, Emotional Cutoff, and Fusion subscales of the Differentiation of Self Inventory (Skowron & Friedlander, 1998) and the Workplace Differentiation Inventory developed by the authors assessed differentiation of self in the workplace. As hypothesized, participants who scored as more highly differentiated on both scales reported greater overall job satisfaction and lower interpersonal stress. Researchers found significant positive correlations between the subscales of the Differentiation of Self Inventory and the Workplace Differentiation Inventory, which suggests that workers tend to respond to interpersonal workplace situations in similar ways as their responses to other relationships. Researchers found significant gender differences, with women reporting more Emotional Reactivity and Fusion than men do. The authors use Bowen's theory of differentiation in discussing these results.