Reliability and robustness of simultaneous absolute quantification of drug transporters, cytochrome P450 enzymes, and Udp-glucuronosyltransferases in human liver tissue by multiplexed MRM/selected reaction monitoring mode tandem mass spectrometry with nano-liquid chromatography

Authors

  • Atsushi Sakamoto,

    Corresponding author
    1. Nippon Boehringer Ingelheim, Pharmacokinetics and Nonclinical Safety, Kobe, Hyogo 650-0047, Japan
    2. Division of Membrane Transport and Drug Targeting, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan
    • Nippon Boehringer Ingelheim, Pharmacokinetics and Nonclinical Safety, Kobe, Hyogo 650-0047, Japan. Telephone: +81-78-306-4527; Fax: +81-78-306-1437
    Search for more papers by this author
  • Takehisa Matsumaru,

    1. Nippon Boehringer Ingelheim, Pharmacokinetics and Nonclinical Safety, Kobe, Hyogo 650-0047, Japan
    Search for more papers by this author
  • Naoki Ishiguro,

    1. Nippon Boehringer Ingelheim, Pharmacokinetics and Nonclinical Safety, Kobe, Hyogo 650-0047, Japan
    Search for more papers by this author
  • Olaf Schaefer,

    1. Nippon Boehringer Ingelheim, Pharmacokinetics and Nonclinical Safety, Kobe, Hyogo 650-0047, Japan
    Search for more papers by this author
  • Sumio Ohtsuki,

    1. Division of Membrane Transport and Drug Targeting, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan
    Search for more papers by this author
  • Tae Inoue,

    1. Division of Membrane Transport and Drug Targeting, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan
    Search for more papers by this author
  • Hirotaka Kawakami,

    1. Division of Membrane Transport and Drug Targeting, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan
    Search for more papers by this author
  • Tetsuya Terasaki

    1. Division of Membrane Transport and Drug Targeting, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan
    Search for more papers by this author

Abstract

Mass spectrometry (MS)-based multiplexed multiple reaction monitoring quantification of proteins has recently evolved as a versatile tool for accurate, absolute quantification of proteins. The purpose of this study was to examine the validity of the present method with regard to standard bioanalytical criteria for drug transporters, cytochrome P450 (CYP) enzymes and uridine 5′-diphospho-glucuronosyltransferases (UGTs). Membrane preparations from human liver tissue were used for target protein quantification. As a result, the determination coefficients (r2) of all targets were greater than 0.986. In the absence of matrix, inaccuracy values (expressed as % deviation) were −8.1% to 20.3%, whereas imprecision values (expressed as % coefficient of variation) were within 15.9%. In the presence of matrix, which consisted of digested plasma membrane fraction for transporters and digested microsomal membrane fraction for CYP enzymes and UGTs, respectively, the inaccuracy was −15.3%–8.1%, and the imprecision were within 18.9%. Sufficient sample stability of membrane fraction was shown for three freeze–thaw cycles, 32 days at −20°C, and in processed samples for 7 days at 10°C. In conclusion, this study demonstrated, for the first time, that the MS-based assay with nano-liquid chromatography provides adequate reliability and robustness for the quantification of selected drug transporters, P450 enzymes and UGTs. © 2011 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 100:4037–4043, 2011

Ancillary