Analysis of transcutaneous antigenic protein delivery by a hydrogel patch formulation

Authors

  • Kazuhiko Matsuo,

    1. Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan
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  • Yumiko Ishii,

    1. Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan
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  • Yasuaki Kawai,

    1. Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan
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  • Yuki Saiba,

    1. Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan
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  • Ying-Shu Quan,

    1. CosMED Pharmaceutical Company Ltd., Minami-ku, Kyoto 601-8014, Japan
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  • Fumio Kamiyama,

    1. CosMED Pharmaceutical Company Ltd., Minami-ku, Kyoto 601-8014, Japan
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  • Sachiko Hirobe,

    1. Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan
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  • Naoki Okada,

    Corresponding author
    1. Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan
    • Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan. Telephone: +81-6-6879-8176; Fax: +81-6-6879-8176
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  • Shinsaku Nakagawa

    Corresponding author
    1. Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan
    • Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan. Telephone: +81-6-6879-8175; Fax: +81-6-6879-8179
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Abstract

We have developed a hydrogel patch, which could promote antigen penetration through stratum corneum (SC), and have demonstrated its safety and efficacy in animals and humans. For the availability improvement of our system, it is important to develop a device, which enhances antigen penetration through SC more efficiently. In this study, we have tried to collect the basic information involved in transcutaneous antigen delivery by investigating the immune event induced by our system and examining the effect of physical property of antigens or patch component on antigen penetration. A hydrogel patch delivered antigens through SC into skin, and some of Langerhans cells captured antigens, activated, and migrated to regional lymph nodes. We also showed that protein distribution into SC was regulated by various complexly-intertwined factors of proteins but not one particular parameter. Additionally, glycerin as the patch component contributed to the formation of SC hydration by patch application, which might be one of the factors of acceleration of protein penetration. On the basis of the present information, we are planning to modify the patch composition and establish the antigen modification technology for improvement in the efficacy of transcutaneous immunization. © 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 102:1936–1947, 2013

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