Quantitative separation and estimation of steroid mixtures by thin-layer chromatography II. Determination of progesterone and estradiol benzoate and of progesterone, testosterone propionate, and estradiol benzoate in mixtures

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Abstract

Rapid and simple procedures were developed for the TLC separation and subsequent quantitative determination of progesterone and estradiol benzoate as well as progesterone, testosterone propionate, and estradiol benzoate in vegetable oil solutions and microcrystalline water suspensions. The solvent system proposed permits not only a rapid quantitative separation of the steroids from one another but at the same time enables the separation of the steroids from the vegetable oil. No special extraction of steroids from the oil is thus necessary. The separated steroids after extraction from the adsorbent are each determined by the colorimetric and fluorimetric method, respectively.

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