Surface-enhanced Raman scattering for medical diagnostics and biological imaging
Article first published online: 4 JUL 2005
Copyright © 2005 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Special Issue: Surface enhanced raman spectroscopy
Volume 36, Issue 6-7, pages 640–647, June - July 2005
How to Cite
Vo-Dinh, T., Yan, F. and Wabuyele, M. B. (2005), Surface-enhanced Raman scattering for medical diagnostics and biological imaging. J. Raman Spectrosc., 36: 640–647. doi: 10.1002/jrs.1348
- Issue published online: 4 JUL 2005
- Article first published online: 4 JUL 2005
- Manuscript Accepted: 15 FEB 2005
- Manuscript Received: 6 NOV 2004
- Office of Biological and Environmental Research, US Department of Energy. Grant Number: DE-AC05-00OR22725.
- surface-enhanced Raman scattering;
- gene detection;
- cellular imaging;
- nanostructured materials
We present recent development and applications of a surface-enhanced Raman scattering (SERS) technology for use in medical diagnostics and biological imaging. For medical diagnostics, we use Raman-active dye-labeled DNA gene probes and nanostructured metallic substrates as SERS-active platforms. The surface-enhanced Raman gene probes can be used to detect DNA biotargets (e.g. gene sequences, bacteria and viral DNA) via hybridization to DNA sequences complementary to these probes. The SERS gene probes eliminate the need for radioactive labels and have great potential to provide both sensitivity, selectivity and label multiplexing for DNA sequencing and clinical assays. We also describe a hyperspectral surface-enhanced Raman imaging (HSERI) system that combines imaging capabilities with SERS detection to identify cellular components with high spatial and temporal resolution. The HSERI system's application to biological imaging is demonstrated using Raman dye-labeled silver nanoparticles in cellular systems. Copyright © 2005 John Wiley & Sons, Ltd.