1097-4555/asset/cover.gif?v=1&s=d2563a28f6ee44d20c855dfca1ce107304920493 "Journal of Raman Spectroscopy")
This work was carried out at the Max-Delbrück-Centrum für Molekulare Medizin Berlin-Buch, Berlin, Robert-Rössle-Str. 10, D-13092 Berlin, Germany.
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Research Article
DNA structure at low pH values, in the presence of Mn2+ ions: a Raman study
Article first published online: 31 AUG 2005
DOI: 10.1002/jrs.1404
Copyright © 2005 John Wiley & Sons, Ltd.
Additional Information
How to Cite
Muntean, C. M., Dostál, L., Misselwitz, R. and Welfle, H. (2005), DNA structure at low pH values, in the presence of Mn2+ ions: a Raman study. J. Raman Spectrosc., 36: 1047–1051. doi: 10.1002/jrs.1404
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This work was carried out at the Max-Delbrück-Centrum für Molekulare Medizin Berlin-Buch, Berlin, Robert-Rössle-Str. 10, D-13092 Berlin, Germany.
Publication History
- Issue published online: 25 OCT 2005
- Article first published online: 31 AUG 2005
- Manuscript Accepted: 22 JUN 2005
- Manuscript Received: 17 MAY 2005
- Abstract
- References
- Cited By
Keywords:
- DNA protonation;
- difference Raman spectroscopy;
- difference spectra;
- calf thymus DNA
Abstract
Raman spectra of calf thymus DNA were measured in the pH interval 6.4 to 3.45 in the presence of divalent manganese ions. pH-dependent protonation of AT and GC base pairs and conformational changes were indicated in the spectra. Protonation of adenine residues becomes obvious at pH 4.4 and continues upon lowering the pH to 3.45. Adenine protonation is connected with the disruption of AT base pairs. Protonation of GC base pairs is indicated at somewhat lower pH than that of AT base pairs, namely at pH 3.8, and continues upon lowering the pH to 3.45. At pH 3.8 unstacking of thymine residues is indicated, and spectral markers for the unstacking of adenine and cytosine were found at pH 3.45. Changes of the DNA backbone are indicated by spectral changes of conformational marker bands at 898 and 1423 cm−1. Copyright © 2005 John Wiley & Sons, Ltd.