Research Article

Surface-enhanced Raman probing of biomolecules using Ag-coated porous glass-ceramic substrates

  1. Z. Pan1,*,
  2. S. H. Morgan1,
  3. A. Ueda1,
  4. R. Mu1,
  5. Y. Cui1,
  6. M. Guo1,
  7. A. Burger1,
  8. Yin Yeh2

Article first published online: 19 SEP 2005

DOI: 10.1002/jrs.1412

Issue

Journal of Raman Spectroscopy

Journal of Raman Spectroscopy

Volume 36, Issue 11, pages 1082–1087, November 2005

Additional Information

How to Cite

Pan, Z., Morgan, S. H., Ueda, A., Mu, R., Cui, Y., Guo, M., Burger, A. and Yeh, Y. (2005), Surface-enhanced Raman probing of biomolecules using Ag-coated porous glass-ceramic substrates. J. Raman Spectrosc., 36: 1082–1087. doi: 10.1002/jrs.1412

Author Information

  1. 1

    Department of Physics, Fisk University, Nashville, TN 37208, USA

  2. 2

    Department of Applied Science, University of California, Davis, CA 95616, USA

*Department of Physics, Fisk University, Nashville, TN 37208, USA.

Publication History

  1. Issue published online: 25 OCT 2005
  2. Article first published online: 19 SEP 2005
  3. Manuscript Accepted: 24 JUN 2005
  4. Manuscript Received: 15 FEB 2005

Funded by

  • NIH/NIGMS. Grant Number: 1S06-GM62813-01.
  • NSF Center for Biophotonics. Grant Number: CA No. PHY 0120999.

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (158K)

Keywords:

  • surface-enhanced Raman scattering (SERS);
  • glass-ceramic;
  • substrate;
  • amino acid;
  • antifreeze glycoprotein

Abstract

Surface-enhanced Raman scattering (SERS) of an amino acid, phenylalanine and an antifreeze glycoprotein have been probed using Ag-coated porous glass-ceramic substrates. The pore size of porous glass-ceramic was in the 50-nm to submicrometer range. The test molecules in aqueous solution were directly adsorbed onto porous glass-ceramic and dried out. The samples were then subsequently coated with an Ag overlayer film of 50 Å in mass thickness. Surface-enhanced Raman spectra of phenylalanine and antifreeze glycoprotein were obtained, and they showed Raman signatures with certain changes compared to those of regular Raman spectra from aqueous solutions and solid samples. The results suggest that the phenylalanine and antifreeze glycoprotein molecules were chemisorbed onto substrate and interacted with Ag film for enhanced Raman scattering. Copyright © 2005 John Wiley & Sons, Ltd.

Get PDF (158K)