Raman spectroscopy shows antifreeze glycoproteins interact with highly oriented pyrolytic graphite
Version of Record online: 19 SEP 2005
Copyright © 2005 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 36, Issue 12, pages 1113–1117, December 2005
How to Cite
Cui, Y., Turner, G., Roy, U. N., Guo, M., Pan, Z., Morgan, S., Burger, A. and Yeh, Y. (2005), Raman spectroscopy shows antifreeze glycoproteins interact with highly oriented pyrolytic graphite. J. Raman Spectrosc., 36: 1113–1117. doi: 10.1002/jrs.1415
- Issue online: 27 NOV 2005
- Version of Record online: 19 SEP 2005
- Manuscript Accepted: 24 JUN 2005
- Manuscript Received: 15 APR 2005
- MBRS program. Grant Number: 1S06-GM62813-01.
- NSF/HRD. Grant Number: 0420516.
- NSF Center for Biophotonics. Grant Number: PHY 0120999.
- antifreeze glycoproteins;
- highly oriented pyrolytic graphite;
Raman spectra of a mixture of antifreeze glycoproteins (AFGP) 6, 7 and 8 adsorbed on a highly oriented pyrolytic graphite (HOPG) surface spanning the range of 50 to 4500 cm−1 were measured. It was found that the Raman signature of AFGP had been significantly altered because of its interaction with the HOPG surface; when compared with the spectra of bulk AFGP compounds, at least three new peaks were observed around 860, 940 and 1000 cm−1, and two other peaks that were not well resolved in the bulk spectra around 1050 and 1155 cm−1 were enhanced and well discriminated. The peak positions depended on the facet of HOPG presented to the proteins, and their relative intensities depended on the concentration of AFGP used in the adsorption experiment. The results indicated the possibility of multiple binding sites of AFGP on HOPG surfaces. Copyright © 2005 John Wiley & Sons, Ltd.