Presented as part of a commemorative issue for Wolfgang Kiefer on the occasion of his 65th birthday.
Raman and infrared spectroscopic mapping of human primary intracranial tumors: a comparative study†
Article first published online: 12 JAN 2006
Copyright © 2006 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Special Issue: Commemorative Issue: for Wolfgang Kiefer on the Occasion of his 65th Birthday
Volume 37, Issue 1-3, pages 367–375, January - March 2006
How to Cite
Krafft, C., Sobottka, S. B., Schackert, G. and Salzer, R. (2006), Raman and infrared spectroscopic mapping of human primary intracranial tumors: a comparative study. J. Raman Spectrosc., 37: 367–375. doi: 10.1002/jrs.1450
- Issue published online: 12 JAN 2006
- Article first published online: 12 JAN 2006
- Manuscript Accepted: 19 JUL 2005
- Manuscript Received: 12 APR 2005
- Volkswagen Foundation.
- near infrared Raman spectroscopy;
- infrared microspectroscopy;
- spectral mapping;
- tumor diagnostics
Intracranial tumors are neoplasias of brain tissue or other tissue inside the skull. Cryosections of the three most frequent primary intracranial tumors—gliomas, meningeomas and schwannomas—were prepared on calcium fluoride windows and studied by both Raman spectroscopy and infrared (IR) spectroscopy. Spectroscopic maps were recorded by sequential acquisition of spectra in a raster pattern. Cluster analyses on selected wavenumber regions were applied for evaluation of these data sets. Raman spectroscopic contributions of proteins including collagen and hemoglobin were identified in cluster centroids, as were nucleic acids and lipids including cholesterol, cholesterol ester (CE) and phosphatidylcholine (PC). Moreover, hydroxyapatite and tricalciumphosphate could be identified as markers for calcification. The spatial distributions of these spectral properties were visualized in pseudocolor Raman and IR maps representing the cluster memberships. The prospects of both methods are discussed. Copyright © 2006 John Wiley & Sons, Ltd.