Presented as part of a commemorative issue for Wolfgang Kiefer on the occasion of his 65th birthday.
Surface- and tip-enhanced Raman scattering of DNA components†
Article first published online: 12 JAN 2006
Copyright © 2006 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Special Issue: Commemorative Issue: for Wolfgang Kiefer on the Occasion of his 65th Birthday
Volume 37, Issue 1-3, pages 311–317, January - March 2006
How to Cite
Rasmussen, A. and Deckert, V. (2006), Surface- and tip-enhanced Raman scattering of DNA components. J. Raman Spectrosc., 37: 311–317. doi: 10.1002/jrs.1480
- Issue published online: 12 JAN 2006
- Article first published online: 12 JAN 2006
- Manuscript Accepted: 13 SEP 2005
- Manuscript Received: 29 JUN 2005
- Alexander von Humboldt Stiftung.
- ZIP program of the German federal government.
- near-field optics;
- tip-enhanced Raman scattering (TERS);
- pyrimidine bases
While surface-enhanced Raman scattering (SERS) is an established technique to significantly enhance the Raman signal from samples bound to metal surfaces, a quantitative interpretation is very difficult because of the rough and arbitrary nature of the substrate. A distinction between concentration and enhancement effects is virtually impossible. Tip-enhanced Raman scattering (TERS), a scattering type of near-field optical microscopy technique, circumvents the problem of random enhancement by concentrating the roughness to only a small region directly at the apex of a scanning probe tip. In this paper, TERS spectra of DNA pyrimidine bases have been collected and compared with standard SERS and Raman measurements of the nucleotides and the pure bases. The results are discussed in terms of electromagnetic and chemical effects of the SERS enhancement of the TERS probes. Copyright © 2006 John Wiley & Sons, Ltd.