Raman spectroscopic study of the heterogeneity of microcolonies of a pigmented bacterium
Article first published online: 13 MAR 2006
Copyright © 2006 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 37, Issue 9, pages 932–936, September 2006
How to Cite
Goodwin, J. R., Hafner, L. M. and Fredericks, P. M. (2006), Raman spectroscopic study of the heterogeneity of microcolonies of a pigmented bacterium. J. Raman Spectrosc., 37: 932–936. doi: 10.1002/jrs.1523
- Issue published online: 25 AUG 2006
- Article first published online: 13 MAR 2006
- Manuscript Accepted: 8 JAN 2006
- Manuscript Received: 22 SEP 2005
- bacterial colony;
- Raman microspectroscopy;
Small colonies (90–350 µm in diameter) of the pigmented halophilic bacterium Halobacterium salinarium have been studied by Raman microspectroscopy using excitation at 632.8 nm. The Raman spectra were dominated by bands due to carotenoid compounds. The intensity of the pigment bands varied across the diameter of the colonies in a way that suggested the presence of concentric rings of higher pigment concentration. Although the colonies were grown in a transparent incubator in an open laboratory, it was demonstrated that the rings were not a result of the diurnal variation of light and dark. These results support a proposed model relating cell motility and cell concentration. Copyright © 2006 John Wiley & Sons, Ltd.