The investigation of single bacteria by means of fluorescence staining and Raman spectroscopy
Article first published online: 12 MAR 2007
Copyright © 2007 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 38, Issue 4, pages 369–372, April 2007
How to Cite
Krause, M., Radt, B., Rösch, P. and Popp, J. (2007), The investigation of single bacteria by means of fluorescence staining and Raman spectroscopy. J. Raman Spectrosc., 38: 369–372. doi: 10.1002/jrs.1721
- Issue published online: 22 MAR 2007
- Article first published online: 12 MAR 2007
- Manuscript Accepted: 19 JAN 2007
- Manuscript Received: 12 JAN 2007
- Carl Zeiss AG
- Raman spectroscopy;
- fluorescence staining;
The successful combination of Raman spectroscopy and fluorescence labelling of single bacterial cells is presented. The results show that the selected dyes (DAPI, SYTO 9, CFDA, SYTOX blue and PI) in most cases do not influence the Raman spectrum of a labelled bacterium significantly compared to an unstained bacterium spectrum. The labelling of bacteria offers the possibility of live/dead or biotic/abiotic differentiation prior to a Raman identification step. Copyright © 2007 John Wiley & Sons, Ltd.