Reference database of Raman spectra of biological molecules
Article first published online: 11 APR 2007
Copyright © 2007 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 38, Issue 9, pages 1133–1147, September 2007
How to Cite
De Gelder, J., De Gussem, K., Vandenabeele, P. and Moens, L. (2007), Reference database of Raman spectra of biological molecules. J. Raman Spectrosc., 38: 1133–1147. doi: 10.1002/jrs.1734
- Issue published online: 22 AUG 2007
- Article first published online: 11 APR 2007
- Manuscript Accepted: 31 JAN 2007
- Manuscript Received: 15 DEC 2006
- Research Foundation-Flanders (F.W.O.-Vlaanderen)
- Research Council UGent (B.O.F.)
- Raman spectroscopy;
- reference Raman spectra
Raman spectra of biological materials are very complex, because they consist of signals from all molecules present in cells. In order to obtain chemical information from these spectra, it is necessary to know the Raman patterns of the possible components of a cell. In this paper, we present a collection of Raman spectra of biomolecules that can serve as references for the interpretation of Raman spectra of biological materials. We included the most important components present in a cell: (1) DNA and RNA bases (adenine, cytosine, guanine, thymine and uracil), (2) amino acids (glycine, L-alanine, L-valine, L-serine, L-glutamic acid, L-arginine, L-phenylalanine, L-tyrosine, L-tryptophan, L-histidine, L-proline), (3) fatty acids and fats (lauric acid, myristic acid, palmitic acid, stearic acid, 12-methyltetradecanoic acid, 13-methylmyristic acid, 14-methylpentadecanoic acid, 14-methylhexadecanoic acid, 15-methylpalmitic acid, oleic acid, vaccenic acid, glycerol, triolein, trilinolein, trilinolenin), (4) saccharides (β-D-glucose, lactose, cellulose, D-(+)-dextrose, D-(+)-trehalose, amylose, amylopectine, D-(+)-mannose, D-(+)-fucose, D-(−)-arabinose, D-(+)-xylose, D-(−)-fructose, D-(+)-galactosamine, N-acetyl-D-glucosamine, chitin), (5) primary metabolites (citric acid, succinic acid, fumarate, malic acid, pyruvate, phosphoenolpyruvate, coenzyme A, acetyl coenzyme A, acetoacetate, D-fructose-6-phosphate) and (6) others (β-carotene, ascorbic acid, riboflavin, glutathione). Examples of Raman spectra of bacteria and fungal spores are shown, together with band assignments to the reference products. Copyright © 2007 John Wiley & Sons, Ltd.