Carotenes and carotenoids in natural biological samples: a Raman spectroscopic analysis
Article first published online: 19 OCT 2009
Copyright © 2009 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 41, Issue 6, pages 642–650, June 2010
How to Cite
de Oliveira, V. E., Castro, H. V., Edwards, H. G. M. and de Oliveira, L. F. C. (2010), Carotenes and carotenoids in natural biological samples: a Raman spectroscopic analysis. J. Raman Spectrosc., 41: 642–650. doi: 10.1002/jrs.2493
- Issue published online: 11 JUN 2010
- Article first published online: 19 OCT 2009
- Manuscript Accepted: 18 AUG 2009
- Manuscript Received: 20 MAY 2009
- FAPEMIG. Grant Number: CEX 1806-07
- Raman spectroscopy;
- plant tissue;
- polyene conjugation
Raman spectroscopic studies of a range of naturally occurring carotenoids in over 50 specimens of plant tissue and a range of standard extracts have been undertaken, and the characteristic bands of CC and CC stretching and CCH bending have been recorded. Comparison of the spectroscopic data with the chemical assignment of the carotenoids from chemical extraction of the plant tissues reveals that there is a problem in the interpretation of the spectroscopic data which can be attributed to significant wavenumber shifts, particularly in the CC stretching band wavenumber, for carotenoids in the organic tissues arising from molecular interactions between the carotenoid and its host matrix. The simple identification of carotenoids in biological tissues on the basis of comparison with the standard spectra of extracted material must be made with caution; the progressive shift in wavenumber of the CC stretching band in the conjugated polyene chain of carotenoids with the number of CC groups, and hence the identification of the carotenoid, cannot be unambiguously interpreted for the range of materials studied here. Copyright © 2009 John Wiley & Sons, Ltd.