Surface-enhanced Raman spectroscopy for quantitative measurement of lactic acid at physiological concentration in human serum
Article first published online: 25 FEB 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 41, Issue 12, pages 1610–1614, December 2010
How to Cite
Hsu, P.-H. and Chiang, H. K. (2010), Surface-enhanced Raman spectroscopy for quantitative measurement of lactic acid at physiological concentration in human serum. J. Raman Spectrosc., 41: 1610–1614. doi: 10.1002/jrs.2609
- Issue published online: 16 DEC 2010
- Article first published online: 25 FEB 2010
- Manuscript Accepted: 8 DEC 2009
- Manuscript Received: 23 FEB 2009
- lactic acid;
- surface-enhanced Raman spectroscopy (SERS);
- silver colloidal nanoparticles;
- cutoff value (5 kDa)
Lactic acid is a simple and effective indicator for estimating physiological function. Rapid and sensitive detection of lactic acid is very useful in clinical diagnosis. However, the concentration of lactic acid in the physiological state is too low to be detected using traditional Raman spectroscopy. We applied silver colloidal nanoparticles-mediated surface-enhanced Raman spectroscopy (SERS) for rapid identification and quantification of lactic acid. The standard SERS spectra of lactic acid were defined and the 1395 cm−1 band intensity was used for quantification from 0.3 to 2 mM (R2 = 0.99). In clinical blood sample measurement, the ultrafiltration (cutoff value 5 kDa) can efficiently reduce background fluorescence to improve SERS performance. We established identical and optimal procedure by adjusting reaction time and volume ratio of serum and nanoparticles to obtain high SERS reproducibility. Finally, we showed that silver colloidal nanoparticles-mediated SERS technique was successfully applied to detect lactic acid at physiological concentrations in the blood. Copyright © 2010 John Wiley & Sons, Ltd.