Chemical imaging of glucose by CARS microscopy
Article first published online: 19 FEB 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 41, Issue 12, pages 1638–1644, December 2010
How to Cite
Åkeson, M., Brackmann, C., Gustafsson, L. and Enejder, A. (2010), Chemical imaging of glucose by CARS microscopy. J. Raman Spectrosc., 41: 1638–1644. doi: 10.1002/jrs.2619
- Issue published online: 16 DEC 2010
- Article first published online: 19 FEB 2010
- Manuscript Accepted: 20 DEC 2009
- Manuscript Received: 24 NOV 2009
- Swedish Research Council
- coherent anti-Stokes Raman scattering (CARS);
- CARS microscopy;
Glucose is one of the most fundamental molecules within life and bioengineering sciences. Present understanding of its role in cellular and bioengineering processes relies primarily on invasive, large-scale biochemical analysis, providing no spatial information on glucose pools or fluxes. This work identifies an emerging microscopy technique based on coherent anti-Stokes Raman scattering (CARS), which fulfills the need of quantitative imaging of glucose at the single-cell level with submicrometer resolution. No sample preparation with reporter molecules is required, ensuring that the low-weight metabolite is studied under natural conditions. The potential of CARS microscopy is illustrated by quantitatively mapping glucose fluxes and distributions in a microfluidic bioreactor and in lipid-bilayer vesicles, the latter as a model for glucose transmembrane transport. Furthermore, the metabolic response to a glucose pulse was monitored in living yeast cells. This study signifies a new era within CARS microscopy for its use of monitoring carbohydrates, in particular glucose which is one of the most abundant molecules in nature. Copyright © 2010 John Wiley & Sons, Ltd.