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Depth-resolved in vivo micro-Raman spectroscopy of a murine skin tumor model reveals cancer-specific spectral biomarkers

Authors

  • Hequn Wang,

    1. Cancer Imaging Department, British Columbia Cancer Research Centre, Vancouver, BC, Canada V5Z 1L3
    2. Laboratory for Advanced Medical Photonics, Photomedicine Institute, Department of Dermatology and Skin Science, University of British Columbia and Vancouver Coastal Health Research Institute, 835 West 10th Avenue, Vancouver, B.C., Canada V5Z 4E8
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  • Naiyan Huang,

    1. Cancer Imaging Department, British Columbia Cancer Research Centre, Vancouver, BC, Canada V5Z 1L3
    2. Laboratory for Advanced Medical Photonics, Photomedicine Institute, Department of Dermatology and Skin Science, University of British Columbia and Vancouver Coastal Health Research Institute, 835 West 10th Avenue, Vancouver, B.C., Canada V5Z 4E8
    3. Department of Laser Medicine, Chinese PLA General Hospital, Beijing 100853, China
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  • Jianhua Zhao,

    1. Cancer Imaging Department, British Columbia Cancer Research Centre, Vancouver, BC, Canada V5Z 1L3
    2. Laboratory for Advanced Medical Photonics, Photomedicine Institute, Department of Dermatology and Skin Science, University of British Columbia and Vancouver Coastal Health Research Institute, 835 West 10th Avenue, Vancouver, B.C., Canada V5Z 4E8
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  • Harvey Lui,

    1. Cancer Imaging Department, British Columbia Cancer Research Centre, Vancouver, BC, Canada V5Z 1L3
    2. Laboratory for Advanced Medical Photonics, Photomedicine Institute, Department of Dermatology and Skin Science, University of British Columbia and Vancouver Coastal Health Research Institute, 835 West 10th Avenue, Vancouver, B.C., Canada V5Z 4E8
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  • Mladen Korbelik,

    1. Cancer Imaging Department, British Columbia Cancer Research Centre, Vancouver, BC, Canada V5Z 1L3
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  • Haishan Zeng

    Corresponding author
    1. Cancer Imaging Department, British Columbia Cancer Research Centre, Vancouver, BC, Canada V5Z 1L3
    2. Laboratory for Advanced Medical Photonics, Photomedicine Institute, Department of Dermatology and Skin Science, University of British Columbia and Vancouver Coastal Health Research Institute, 835 West 10th Avenue, Vancouver, B.C., Canada V5Z 4E8
    • Cancer Imaging Department, BC Cancer Research Center, 675 West 10th Avenue, Vancouver, BC, Canada V5Z1L3.
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Abstract

We have developed a micro-Raman spectrometer system for use to differentiate tumor lesions from normal skin using an in vivo animal model. A study of 494 Raman spectra from 24 mice revealed different spectral patterns at different depths and between normal and tumor-bearing skin sites. A peak at 899 cm−1 (possibly from proline or fatty acids) and one with higher intensity in the 1325–1330 cm−1 range (assigned to nucleic acids) were correlated with the presence of tumors, which can potentially be used as biomarkers for skin cancer detection. Spectral diagnosis performed on the murine tumor model achieved a diagnostic sensitivity of 95.8% and specificity of 93.8%. These results encourage us to develop further the use of confocal Raman spectroscopy as a clinical tool for noninvasive human skin biochemical analysis, particularly in relation to skin cancer. Copyright © 2010 John Wiley & Sons, Ltd.

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