Raman spectroscopic study on sodium hyaluronate: an effect of proton and γ irradiation

Authors

  • Alla Synytsya,

    Corresponding author
    1. Department of Analytical Chemistry, Institute of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic
    • Department of Analytical Chemistry, Institute of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic.
    Search for more papers by this author
  • Andriy Synytsya,

    1. Department of Carbohydrate Chemistry and Technology, Institute of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic
    Search for more papers by this author
  • Petr Alexa,

    1. Institute of Physics, VŠB, Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava, Czech Republic
    Search for more papers by this author
  • Richard Wagner,

    1. Department of Radiation Dosimetry, Nuclear Physics Institute ASCR v.v.i., Na Truhlarce 39/64, 18086 Prague 8, Czech Republic
    Search for more papers by this author
  • Marie Davídková,

    1. Department of Radiation Dosimetry, Nuclear Physics Institute ASCR v.v.i., Na Truhlarce 39/64, 18086 Prague 8, Czech Republic
    Search for more papers by this author
  • Karel Volka

    1. Department of Analytical Chemistry, Institute of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic
    Search for more papers by this author

Abstract

Raman spectroscopy was applied to the analysis of structural changes in lyophilised sodium hyaluronate after proton and γ irradiation (0.5, 5, 50, 100, 200 and 600 Gy). Characteristic Raman bands of the polysaccharide were sensitive to irradiation. Significant damage was observed at doses of 50 Gy or higher. The spectral changes confirmed radiation-induced loss of native solution conformation, destruction of primary structure, fragmentation, cross-linking and elimination of functional groups. Differences in the effects of proton and γ radiation on sodium hyaluronate are discussed. Copyright © 2010 John Wiley & Sons, Ltd.

Ancillary