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Optical sectioning using single-plane-illumination Raman imaging

  1. Ishan Barman1,*,
  2. Khay Ming Tan2,
  3. Gajendra Pratap Singh2,*

Article first published online: 26 SEP 2010

DOI: 10.1002/jrs.2785

Issue

Journal of Raman Spectroscopy

Journal of Raman Spectroscopy

Volume 41, Issue 10, pages 1099–1101, October 2010

Additional Information

How to Cite

Barman, I., Tan, K. M. and Singh, G. P. (2010), Optical sectioning using single-plane-illumination Raman imaging. J. Raman Spectrosc., 41: 1099–1101. doi: 10.1002/jrs.2785

Author Information

  1. 1

    G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

  2. 2

    School of Physics and Astronomy, University of St. Andrews, St. Andrews, Fife, KY16 9SS, UK

*G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Publication History

  1. Issue published online: 11 OCT 2010
  2. Article first published online: 26 SEP 2010
  3. Manuscript Accepted: 21 JUL 2010
  4. Manuscript Received: 14 MAY 2010

Funded by

  • Laser Biomedical Research Centre at MIT

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Keywords:

  • single-plane-illumination microscopy (SPIM);
  • single-plane-illumination Raman imaging (SPIRI);
  • Raman imaging;
  • Raman spectroscopy;
  • optical sectioning

Abstract

Confocal Raman imaging is widely used for optical sectioning of materials. However, for biological applications it often suffers from poor axial resolution, photodamage to the sample of interest, substrate interference, and long acquisition times. We have applied the principles of light sheet microscopy to Raman imaging and show for the first time that optically sectioned Raman images can be obtained in significantly lower acquisition times. Copyright © 2010 John Wiley & Sons, Ltd.

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