Surface-enhanced Raman spectroscopy in living plant using triplex Au[BOND]Ag[BOND]C core–shell nanoparticles

Authors

  • Aiguo Shen,

    1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China
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    • These authors contributed equally to this work.

  • Jingzhe Guo,

    1. Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China
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    • These authors contributed equally to this work.

  • Wei Xie,

    1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China
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  • Mengxiang Sun,

    1. Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China
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  • Ryan Richards,

    1. Department of Chemistry and Geochemistry, Colorado School of Mines, Golden, CO 80401, USA
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  • Jiming Hu

    Corresponding author
    1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China
    • Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
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Abstract

This paper presents, for the first time, noninvasive imaging of a livingplant using biocompatible carbon-encapsulated Au[BOND]Ag nanoparticles (NPs) using micro-Raman spectroscopy (MRS). A convenient and controllable hydrothermal synthetic route was developed to synthesize the layer-by-layer triplex Au[BOND]Ag[BOND]C core–shell NPs, which can incorporate the reporter molecule 4-mercapto benzoic acid (4-MBA). A unique approach was devised to deliver the carbon-encapsulated surface-enhanced Raman scattering (SERS) tags into the leaf of Nicotiana benthamiana. In vivo SERS mapping was subsequently performed to monitor the distribution of tags inside the leaf, which successfully avoided interference of autofluorescence from plant tissue. The imaging modality reported here and further the bio-functionalized carbon-encapsulated SERS NPshold significant potential as a strategy forbiochemical imaging in living plantsin a noninvasive and nontoxic manner, whichmight open up exciting opportunities for plant sciences. Copyright © 2010 John Wiley & Sons, Ltd.

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